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ATF3 (N159) polyclonal antibody

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 Catalog No.Package SizePrice
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Catalog No.BS2261
Product NameATF3 (N159) polyclonal antibody
ApplicationsIHC
Alternative NameCyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3; ATF3; ATF 3
Swiss-ProtP18847
HostRabbit
ReactivityHuman
Application_allIHC: 1:50~1:200
ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
SpecificityATF-3 (N159) polyclonal antibody detects endogenous levels of ATF-3 protein.
BiowMW~ 24 kDa
NoteFor research use only, not for use in diagnostic procedure.
ImmunogenSynthetic peptide, corresponding to amino acids 120-170 of Human ATF3.
  • Western blot (WB) analysis of ATF3 (N159) pAb at 1:500 dilution
    Lane1:L02 whole cell lysate(40ug)
    Lane2:SGC7901 whole cell lysate(40ug)
    Lane3:A549 whole cell lysate(40ug)
    Lane4:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of ATF3 (N159) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Background

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.

References for ATF3 (N159) polyclonal antibody

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