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Histone H3 (Tri-Methyl K9) monoclonal antibody

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Catalog No.MB2026
Product NameHistone H3 (Tri-Methyl K9) monoclonal antibody
ApplicationsWB
Alternative NameH3K9me3
Swiss-ProtP68431
HostMouse
ReactivityHuman, Mouse, Rat
Application_allWB: 1:500~1:2000
ProductPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Purification&PurityThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
SpecificityHistone H3 (Tri-Methyl K9) mAb detects endogenous levels of histone H3 only when Tri-methylated at Lys9.
BiowMW~ 17 kDa
NoteFor research use only, not for use in diagnostic procedure.
ImmunogenSynthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, Tri methylated at K9.
  • Western blot analysis of 1) Hela cells, 2) Rat Testis tissue, 3) Raw264.7 cells with Histone H3 (Tri-Methyl K9) mAb diluted at 1:2000.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.

References for Histone H3 (Tri-Methyl K9) monoclonal antibody

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