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Rel B (phospho-S552) polyclonal antibody

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 Catalog No.Package SizePrice
 BS477050ulenquire
 BS4770100ulenquire
 Catalog No.Package SizePrice
BS477050ul$168
BS4770100ul$298
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Catalog No.BS4770
Product NameRel B (phospho-S552) polyclonal antibody
ApplicationsWB IHC
Alternative NameTranscription factor RelB; I-Rel; RELB
Swiss-ProtQ01201
HostRabbit
ReactivityHuman,Mouse,Rat
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
Product1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificityp-Rel B (S552) polyclonal antibody detects endogenous levels of Rel B protein only when phosphorylated at Ser552.
BiowMW~ 70 kDa
NoteFor research use only, not for use in diagnostic procedure.
ImmunogenSynthetic phosphopeptide derived from human Rel B around the phosphorylation site of Serine 552.
  • Western blot (WB) analysis of p-Rel B (S552) polyclonal antibody at 1:500 dilution
    Lane1:Hela cell lysate treated with TNFa
    Lane2:Mouse stomach tissue lysate
    Lane3:Mouse colon tissuel lysate
    Lane4:Rat colon tissue lysate
  • Immunohistochemistry (IHC) analyzes of p-Rel B (S552) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Western blot (WB) analysis of p-RelB (S552) pAb at 1:500 dilution
    Lane1:PC3 whole cell lysate(40ug)
    Lane2:PC3 treated with PMA (40nM, 3min) whole cell lysate(40ug)
    Lane3:PC3 treated with PMA (40nM, 15min) whole cell lysate(40ug)
    Lane4:PC3 treated with PMA
  • Western blot (WB) analysis of p-RelB (S552) pAb at 1:500 dilution
    Lane1:PC3 whole cell lysate(40ug)
    Lane2:PC3 treated with PMA (1nM, 3min) whole cell lysate(40ug)
    Lane3:PC3 treated with PMA (1nM, 5min) whole cell lysate(40ug)
    Lane4:PC3 treated with PMA (1

Background

The NFκB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein. The DNAbinding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A second protein designated p52 (previously referred to as p49) has been identified that can act as an alternative NFκB subunit. Rel B does not bind with high affinity to NFκB sites, but heterodimers between Rel B and p50 bind with an affinity comparable to that of p50 NFκB homodimers. However, Rel B/p50 heterodimers, in contrast to NFκB heterodimers, transactivates transcription of promotors containing κB binding sites.

References for Rel B (phospho-S552) polyclonal antibody

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