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GSK3β (phospho-S9) polyclonal antibody BS4084
  • Western blot (WB) analysis of GSK3β (phospho-Ser9) pAb at 1:500 dilution Lane1:PMVEC whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(30ug) Lane3:HEK293T treated with Calyculin A(50 nM,30 minutes) whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug)
  • Immunohistochemistry (IHC) analyzes of p-GSK3b (S9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameGSK3β (phospho-S9) polyclonal antibody
Catalog No.BS4084
Swiss-ProtP49841
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IP
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 46 kDa
Alternative NameGlycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B; GSK3beta isoform; Serine/threonine-protein kinase GSK3B;
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylated
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100ul $298
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Product Name :
GSK3β (phospho-S9) polyclonal antibody
Background :
Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-GSK3β (S9) polyclonal antibody detects endogenous levels of GSK3β protein only when phosphorylated at Ser9.
Immunogen :
Synthetic phosphopeptide derived from human GSK3β around the phosphorylation site of Serine 9.
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot (WB) analysis of GSK3β (phospho-Ser9) pAb at 1:500 dilution Lane1:PMVEC whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(30ug) Lane3:HEK293T treated with Calyculin A(50 nM,30 minutes) whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug)
  • Immunohistochemistry (IHC) analyzes of p-GSK3b (S9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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PMCID:    Pubmed No.:24769129 Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice PMCID:    Pubmed No.:24752588 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats PMCID:    Pubmed No.:24337945 Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions PMCID:    Pubmed No.:24504086 Interplay between intergrin-linked kinase and ribonuclease inhibitor affects growth and metastasis of bladder cancer through signaling ILK pathways PMCID:    Pubmed No.:27576342 Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation PMCID:    Pubmed No.:25751394 Dietary cholesterol 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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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