CATSPER1 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CATSPER1 polyclonal antibody Background :
CatSper is an ion transport protein located on the surface of sperm cells. CatSper (for cation channel of sperm) is vital to cAMP-mediated calcium influx in sperm, sperm motility and fertilization. The gene that encodes CatSper maps to human chromosome 11q12.1. The 686 amino acid protein is a single, six-transmembrane-spanning protein of the voltage- dependent Ca2+ channel four-repeat structure and is expressed exclusively in the testis. In CatSper -/- sperm, neither cAMP nor cGMP elicit a significant Ca2+ influx in both mutant and wild-type sperm. CatSper is localized to the sperm flagellum and CatSper -/- mice show sluggish and less directed movements. CatSper is required to penetrate the egg, but is not required for egg activation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CATSPER1 polyclonal antibody detects endogenous levels of CATSPER1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human CATSPER1 Conjugate :
Unconjugated Modification :
Unmodified
CATSPER1 polyclonal antibody Background :
CatSper is an ion transport protein located on the surface of sperm cells. CatSper (for cation channel of sperm) is vital to cAMP-mediated calcium influx in sperm, sperm motility and fertilization. The gene that encodes CatSper maps to human chromosome 11q12.1. The 686 amino acid protein is a single, six-transmembrane-spanning protein of the voltage- dependent Ca2+ channel four-repeat structure and is expressed exclusively in the testis. In CatSper -/- sperm, neither cAMP nor cGMP elicit a significant Ca2+ influx in both mutant and wild-type sperm. CatSper is localized to the sperm flagellum and CatSper -/- mice show sluggish and less directed movements. CatSper is required to penetrate the egg, but is not required for egg activation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CATSPER1 polyclonal antibody detects endogenous levels of CATSPER1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human CATSPER1 Conjugate :
Unconjugated Modification :
Unmodified
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Western blot (WB) analysis of CATSPER1 polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate Lane2:Hela whole cell lysate Lane3:SP2/0 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.