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TORC1 (N-terminus) monoclonal antibody MB0104
  • Western blot detection of TORC1 (N-terminus) antibody in Hela&Jurkat lysates using TORC1 (N-terminus) antibody (1:1000 diluted) .
  • Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
Product NameTORC1 (N-terminus) monoclonal antibody
Catalog No.MB0104
Swiss-ProtQ6UUV9
Host Mouse IgG2b
ReactivityHuman
ApplicationsWB FC ICC IP
Application_allWB: 1:1000ICC: 1:100~300IP: 1:50~200 FC: 1:50~100
BiowMWPredicted band size:69KDa Observed band size:69KDa
Alternative NameKIAA0616; CREB regulated transcription coactivator 1; CREB-regulated transcription coactivator 1; CRTC1; CRTC1; FLJ14027; KIAA0616; MECT 1; Mucoepidermoid carcinoma translocated 1; Mucoepidermoid carcinoma translocated protein 1; TORC-1; TORC1; Transducer of CREB protein 1; Transducer of regulated cAMP response element binding protein 1; Transducer of regulated cAMP response element-binding protein (CREB) 1; Transducer of regulated cAMP response element-binding protein 1; WAMTP1; MECT1; TORC1; WAMTP1
Purification&PurityThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
TORC1 (N-terminus) monoclonal antibody
Background :
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 ´Ser-133´ phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses (By similarity)
Product :
Mouse IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
This antibody detects endogenous levels of TORC1 and does not cross-react with related proteins
Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human TORC1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot detection of TORC1 (N-terminus) antibody in Hela&Jurkat lysates using TORC1 (N-terminus) antibody (1:1000 diluted) .
  • Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
Price/Size :

$368/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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