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Tim3 monoclonal antibody MB9542
  • Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/500 dilution.
  • ICC staining Tim3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product NameTim3 monoclonal antibody
Catalog No.MB9542
Swiss-ProtQ8TDQ0(Human)
Host Mouse
ReactivityHuman
ApplicationsWB ICC IHC FC
Application_allWB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:100-1:200 FC:1:50-1:100
BiowMW33 kDa (Predicted band size)
Alternative NameHAVcr-2, TIMD-3, TIM-3, HAVCR2, TIM3, TIMD3, CD366, FLJ14428, HAVcr-2, Havcr2, HAVR2, Hepatitis A virus cellular receptor 2, Kidney injury molecule 3, KIM 3, KIM3, T cell immunoglobulin and mucin domain containing 3, T cell immunoglobulin mucin 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim 3, TIM-3, TIM3, TIMD-3, TIMD3,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
Tim3 monoclonal antibody
Background :
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse.
Product :
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Specificity :
Tim3 monoclonal antibody detects endogenous levels of Tim3 protein.
Immunogen :
Recombinant protein
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/500 dilution.
  • ICC staining Tim3 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Price/Size :

$368/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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