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Zscan4 monoclonal antibody MB9586
  • Western blot analysis of Zscan4 on 293 transfected Zscan4 (1) and 293T (2) cell lysates using anti-Zscan4 antibody at 1/500 dilution.
  • Flow cytometric analysis of HepG2 cells with Zscan4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Product NameZscan4 monoclonal antibody
Catalog No.MB9586
Swiss-ProtQ8NAM6(Human)
Host Mouse
ReactivityHuman
ApplicationsWB FC
Application_allWB:1:500 FC:1:50-1:100
BiowMW49 kDa
Alternative NameZSCAN4, ZNF494, FLJ35105, MGC126787, MGC126789, Zinc finger and SCAN domain containing 4, Zinc finger and SCAN domain containing protein 4, Zinc finger and SCAN domain-containing protein 4, Zinc finger protein 494, ZNF 494, ZNF494, ZSCA4, ZSCAN4,
Purification&PurityProG affinity purified.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
Zscan4 monoclonal antibody
Background :
Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
Product :
Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity :
Zscan4 monoclonal antibody detects endogenous levels of Zscan4 protein.
Immunogen :
Recombinant protein within Human ZSCAN4 aa 1-100.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of Zscan4 on 293 transfected Zscan4 (1) and 293T (2) cell lysates using anti-Zscan4 antibody at 1/500 dilution.
  • Flow cytometric analysis of HepG2 cells with Zscan4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Price/Size :

$368/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

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