Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
Tubulin β polyclonal antibody AP0064
  • Western blot (WB) analysis of Tubulin β pAb at 1:5000/1:10000/1:20000/1:40000 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:MG63 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug) Lane6:The Brain tissue lysate of Rat(40ug) Lane7:The Brain tissue lysate of Mouse(40ug)
  • Western blot (WB) analysis of Tubulin β pAb at 1:5000 dilution Lane1:A549 whole cell lysate(20ug) Lane2:PC12 whole cell lysate(20ug) Lane3:BV2 whole cell lysate(20ug) Lane4:The Kidney tissue lysate of Mouse(20ug) Lane5:The Prostate tissue lysate of Rat(20ug)
Product NameTubulin β polyclonal antibody
Catalog No.AP0064
Swiss-ProtN/A
Host Rabbit
ReactivityHuman,Mouse,Rat(not applicable to mouse tissue)
ApplicationsWB
Application_allWB:1:5000~1:20000
BiowMW~ 55 kDa
Alternative NameTubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; TUBB3; TUBB4
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $148
100ul $258
Add to cart My orders
Product Name :
Tubulin β polyclonal antibody
Background :
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Tubulin beta 1/2/3/5/6/8 polyclonal antibody pAb detects endogenous levels of Tubulin beta 1/2/3/5/6/8 protein.
Immunogen :
Recombinant full length Human Tubulin β.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Tubulin β pAb at 1:5000/1:10000/1:20000/1:40000 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:MG63 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug) Lane6:The Brain tissue lysate of Rat(40ug) Lane7:The Brain tissue lysate of Mouse(40ug)
  • Western blot (WB) analysis of Tubulin β pAb at 1:5000 dilution Lane1:A549 whole cell lysate(20ug) Lane2:PC12 whole cell lysate(20ug) Lane3:BV2 whole cell lysate(20ug) Lane4:The Kidney tissue lysate of Mouse(20ug) Lane5:The Prostate tissue lysate of Rat(20ug)
Protein Kinase C Promotes NMDA Receptor Trafficking by Indirectly Triggering CaMKII Autophosphorylation PMCID:    Pubmed No.:21606495 Reduced neurogenesis and pre-synaptic dysfunction in the olfactory bulb of a rat model of depression PMCID:    Pubmed No.:21722712 Anti-inflammatory and immunomodulatory mechanisms of artemisinin on contact hypersensitivity PMCID:    Pubmed No.:22122827 Relative Expression of Type II MAGE Genes During Retinoic Acid-Induced Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells: A Comparative Real-Time PCR Analysis PMCID:    Pubmed No.:22410673 Reversal effect of Tween-20 on multidrug resistance in tumor cells in vitro PMCID:    Pubmed No.:22440896 MiR-138 suppressed nasopharyngeal carcinoma growth and tumorigenesis by targeting the CCND1 oncogene PMCID:    Pubmed No.:22739938 Implication of PDGF signaling in cigarette smoke-induced pulmonary arterial hypertension in rat PMCID:    Pubmed No.:22746397 Hippocampal Synaptic Dysregulation of Exo/Endocytosis-Associated Proteins Induced in a Chronic Mild Stressed Rat Model PMCID:    Pubmed No.:22922266 Ribose-phosphate pyrophosphokinase 1 (PRPS1) associated with deltamethrin resistance in Culex pipiens pallens PMCID:    Pubmed No.:23250545 Egr-1 enhances drug resistance of breast cancer by modulating MDR1 expression in a GGPPS-independent manner PMCID:    Pubmed No.:23478574 miR-135a/b Modulate Cisplatin Resistance of Human Lung Cancer Cell Line by Targeting MCL1 PMCID:    Pubmed No.:23640248 Direct Blue 71 staining as a destaining-free alternative loading control method for Western blotting PMCID:    Pubmed No.:23712695 Identification of a< i> Bombyx mori gene encoding small heat shock protein BmHsp27. 4 expressed in response to high-temperature stress PMCID:    Pubmed No.:24440242 Toxic effect of zinc nanoscale metal-organic frameworks on Rat Pheochromocytoma (PC12) Cells in vitro PMCID:    Pubmed No.:24637453 Identification of potential pathways involved in the induction of cell cycle arrest and apoptosis by a new 4-arylidene curcumin analogue T63 in lung cancer cells: a comparative proteomic analysis. PMCID:    Pubmed No.:24651282 Oncogenic roles of carbonic anhydrase IX in human nasopharyngeal carcinoma PMCID:    Pubmed No.:25031713 Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis PMCID:    Pubmed No.:25444511 Endostatin Prevents Dietary-Induced Obesity by Inhibiting Adipogenesis and Angiogenesis PMCID:    Pubmed No.:25605807 Dietary Fiber Intake is Associated with Increased Colonic Mucosal GPR43+ Polymorphonuclear Infiltration in Active Crohn's Disease PMCID:    Pubmed No.:26140540 The Effects of HSP27 on Gemcitabine-Resistant Pancreatic Cancer Cell Line Through Snail PMCID:    Pubmed No.:26348464 PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-γ to increase surface FasL PMCID:    Pubmed No.:26519036 Absence of the Adenosine A2A Receptor Confers Pulmonary Arterial Hypertension Through RhoA/ROCK Signaling Pathway in Mice PMCID:    Pubmed No.:26647014 SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer PMCID:    Pubmed No.:26658802 E3 ubiquitin ligase RNF183 is a novel regulator in inflammatory bowel disease PMCID:    Pubmed No.:26818663 Synergistic interaction of effective parts in Rehmanniae Radix and Cornus officinalis ameliorates renal injury in C57BL/KsJ-db/db diabetic mice: Involvement of suppression of AGEs/RAGE/SphK1 signaling pathway. PMCID:    Pubmed No.:26972502 Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis PMCID:    Pubmed No.:25444511 The Effects of HSP27 on Gemcitabine-Resistant Pancreatic Cancer Cell Line Through Snail PMCID:    Pubmed No.:26348464 PEDF and 34-mer inhibit angiogenesis in the heart by inducing tip cells apoptosis via up-regulating PPAR-γ to increase surface FasL PMCID:    Pubmed No.:26519036 Absence of the Adenosine A2A Receptor Confers Pulmonary Arterial Hypertension Through RhoA/ROCK Signaling Pathway in Mice PMCID:    Pubmed No.:26647014 SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer PMCID:    Pubmed No.:26658802 E3 ubiquitin ligase RNF183 is a novel regulator in inflammatory bowel disease PMCID:    Pubmed No.:26818663 Synergistic interaction of effective parts in Rehmanniae Radix and Cornus officinalis ameliorates renal injury in C57BL/KsJ-db/db diabetic mice: Involvement of suppression of AGEs/RAGE/SphK1 signaling pathway. PMCID:    Pubmed No.:26972502 Identification of a< i> Bombyx mori gene encoding small heat shock protein BmHsp27. 4 expressed in response to high-temperature stress PMCID:    Pubmed No.:24440242 Toxic effect of zinc nanoscale metal-organic frameworks on Rat Pheochromocytoma (PC12) Cells in vitro PMCID:    Pubmed No.:24637453 Identification of potential pathways involved in the induction of cell cycle arrest and apoptosis by a new 4-arylidene curcumin analogue T63 in lung cancer cells: a comparative proteomic analysis. PMCID:    Pubmed No.:24651282
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER