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His-tag polyclonal antibody AP0032
  • Western blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 μg of E. coli lysate.
  • \"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
  • \"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
  • \"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
Product NameHis-tag polyclonal antibody
Catalog No.AP0032
Swiss-ProtN/A
Host Rabbit
ReactivityAll
ApplicationsWB IP IF
Application_all\"WB: 1:2000~1:5000 IP: 1:50~1:200 IF: 1:50~1:200\\\"
BiowMWN/A
Alternative Name6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $148
100ul $258
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Product Name :
His-tag polyclonal antibody
Background :
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
His-tag polyclonal antibody detects over-expressed or recombinant proteins containing the 6× His epitope tag
Immunogen :
Synthetic peptide His-tag.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:2000 (lane 1) and 1:5000 (lane 2). Each lane was loaded with 10 μg of E. coli lysate.
  • \"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
  • \"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
  • \"Western blot (WB) analysis of His-tag polyclonal antibody at 1:5000 dillution Lane1:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His.\"
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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