Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
AKT polyclonal antibody AP0059
  • Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Product NameAKT polyclonal antibody
Catalog No.AP0059
Swiss-ProtP31749/P31751/Q9Y243
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
BiowMW~ 60 kDa
Alternative NameRAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $158
100ul $275
Add to cart My orders
Product Name :
AKT polyclonal antibody
Background :
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
AKT polyclonal antibody detects endogenous levels of total AKT protein.
Immunogen :
Recombinant full length Human AKT1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway. PMCID:    Pubmed No.:22139427 Dose-dependent effect of tamoxifen in tamoxifen-resistant breast cancer cells via stimulation by the ERK1/2 and AKT signaling pathways. PMCID:    Pubmed No.:23338782 Activation of cannabinoid type 2 receptor by JWH133 protects heart against ischemia/reperfusion-induced apoptosis. PMCID:    Pubmed No.:23711495 MicroRNA-34a Suppresses Cell Proliferation by Targeting LMTK3 in Human Breast Cancer MCF-7 Cell Line PMCID:    Pubmed No.:24050776 MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2 PMCID:    Pubmed No.:24293274 ART1 Silencing Enhances Apoptosis of Mouse CT26 Cells via the PI3K/Akt/NF-κB Pathway PMCID:    Pubmed No.:24335275 Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway PMCID:    Pubmed No.:24366110 LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells PMCID:    Pubmed No.:24374359 cyclooxygenase-2 inhibitor is a robust enhancer of anticancer agents against hepatocellular carcinoma multicellular spheroids PMCID:    Pubmed No.:24591842 Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway. PMCID:    Pubmed No.:24769129 High uric acid directly inhibits insulin signalling and induces insulin resistance PMCID:    Pubmed No.:24769205 GPER mediates enhanced cell viability and motility via non-genomic signaling induced by 17β-estradiol in triple-negative breast cancer cells PMCID:    Pubmed No.:24874276 The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway PMCID:    Pubmed No.:25530715 A novel Pseudolaric acid B derivative, Hexahydropseudolaric acid B, exterts immunomodulatory effect in vitro/in vivo evaluation PMCID:    Pubmed No.:25446920 Neuroprotection by Kukoamine A against oxidative stress may involve N-methyl-D-aspartate receptors PMCID:    Pubmed No.:25445711 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. PMCID:    Pubmed No.:25410747 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells PMCID:    Pubmed No.:25857968 Frequent amplification of PTP1B is associated with poor survival of gastric cancer patients PMCID:    Pubmed No.:25590580 PM2. 5‐induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF‐κB‐dependent pathway PMCID:    Pubmed No.:25876056 Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway PMCID:    Pubmed No.:26074264 Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy PMCID:    Pubmed No.:26547529 Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway PMCID:    Pubmed No.:26346601 Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo PMCID:    Pubmed No.:26531674 Exogenous avian leukosis virus-induced activation of the ERK/AP1 pathway is required for virus replication and correlates with virus-induced tumorigenesis PMCID:    Pubmed No.:26754177 Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals PMCID:    Pubmed No.:26160845 Heat shock protein 27 downstream of P38-PI3K/Akt signaling antagonizes melatonin-induced apoptosis of SGC-7901 gastric cancer cells PMCID:    Pubmed No.:26877709 Facilitatory effects of fetuin-A on atherosclerosis PMCID:    Pubmed No.:26828753 High Uric Acid Induces Insulin Resistance in Cardiomyocytes In Vitro and In Vivo PMCID:    Pubmed No.:26836389 miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/ AKT pathway in nasopharyngeal carcinoma PMCID:    Pubmed No.:27010857 The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway PMCID:    Pubmed No.:25530715 A novel Pseudolaric acid B derivative, Hexahydropseudolaric acid B, exterts immunomodulatory effect in vitro/in vivo evaluation PMCID:    Pubmed No.:25446920 Neuroprotection by Kukoamine A against oxidative stress may involve N-methyl-D-aspartate receptors PMCID:    Pubmed No.:25445711 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. PMCID:    Pubmed No.:25410747 Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway PMCID:    Pubmed No.:25564356 Ethanolic Ginkgo biloba leaf extract prevents renal fibrosis through Akt/mTOR signaling in diabetic nephropathy PMCID:    Pubmed No.:26547529 Neuroglobin Attenuates Beta Amyloid-Induced Apoptosis Through Inhibiting Caspases Activity by Activating PI3K/Akt Signaling Pathway PMCID:    Pubmed No.:26346601 Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo PMCID:    Pubmed No.:26531674 Exogenous avian leukosis virus-induced activation of the ERK/AP1 pathway is required for virus replication and correlates with virus-induced tumorigenesis PMCID:    Pubmed No.:26754177 Activation of GPER suppresses epithelial mesenchymal transition of triple negative breast cancer cells via NF-κB signals PMCID:    Pubmed No.:26160845 Heat shock protein 27 downstream of P38-PI3K/Akt signaling antagonizes melatonin-induced apoptosis of SGC-7901 gastric cancer cells PMCID:    Pubmed No.:26877709 Facilitatory effects of fetuin-A on atherosclerosis PMCID:    Pubmed No.:26828753 High Uric Acid Induces Insulin Resistance in Cardiomyocytes In Vitro and In Vivo PMCID:    Pubmed No.:26836389 Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway. PMCID:    Pubmed No.:24769129 High uric acid directly inhibits insulin signalling and induces insulin resistance PMCID:    Pubmed No.:24769205 MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2 PMCID:    Pubmed No.:24293274 ART1 Silencing Enhances Apoptosis of Mouse CT26 Cells via the PI3K/Akt/NF-κB Pathway PMCID:    Pubmed No.:24335275 Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway PMCID:    Pubmed No.:24366110 LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells PMCID:    Pubmed No.:24374359 cyclooxygenase-2 inhibitor is a robust enhancer of anticancer agents against hepatocellular carcinoma multicellular spheroids PMCID:    Pubmed No.:24591842
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER