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EKLF (W318) polyclonal antibody BS2303
  • Western blot (WB) analysis of EKLF (W318) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug)
Product NameEKLF (W318) polyclonal antibody
Catalog No.BS2303
Swiss-ProtQ13351
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000IHC: 1:50~1:200IF: 1:50~1:200
BiowMW~ 38 kDa
Alternative NameKrueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF1; EKLF
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
EKLF (W318) polyclonal antibody
Background :
The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF. EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arrest.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
EKLF (W318) polyclonal antibody detects endogenous levels of EKLF protein. This antibody also recognizes lKLF (51 kDa), GKLF (56 kDa), and UKLF (33 kDa).
Immunogen :
Synthetic peptide, corresponding to amino acids 288-335 of Human EKLF.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of EKLF (W318) pAb at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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