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ApoL2 (R229) polyclonal antibody BS3073
  • Western blot (WB) analysis of ApoL2 (R229) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of ApoL2 (R229) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameApoL2 (R229) polyclonal antibody
Catalog No.BS3073
Swiss-ProtQ9BQE5
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000IHC: 1:50~1:200
BiowMW~ 37 kDa
Alternative NameApolipoprotein L2; Apolipoprotein L-2; ApoL-2; APOL2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
ApoL2 (R229) polyclonal antibody
Background :
Apolipoproteins are protein components of plasma lipoproteins. The apolipoprotein L gene family encodes six highly homologous proteins designated apoL 1-6, which are associated with large high density type lipoproteins (HDL). The human apoL family maps to chromosome 22q12.3 within a 127,000-bp region. ApoL2 and apoL3 may allow the binding of lipids to organ-elles or may be involved in the movement of lipids in the cytoplasm. ApoL2 localizes to the cytoplasm and is widely expressed, with the highest levels observed in the lung, thymus, pancreas, placenta, adult brain and prostate. The apoL2 protein is also detected in the spleen, liver, kidney, colon, small intestine, uterus, spinal cord, adrenal gland, salivary gland, trachea, mammary gland, skeletal muscle, testis and fetal brain and liver. ApoL3 also localizes to the cytoplasm and is widely expressed, with highest levels detected in the prostate, lung and placenta. It is also detected in kidney, bone marrow, spleen, thymus, spinal cord, adrenal gland, salivary gland, trachea and mammary gland. Lower levels of apoL3 are observed in the brain, heart, fetal liver, pancreas and testis.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
ApoL2 (R229) polyclonal antibody detects endogenous levels of ApoL2 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 196-240of Human ApoL2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of ApoL2 (R229) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of ApoL2 (R229) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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