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MYOG polyclonal antibody BS8337
  • WesternBlot (WB) analysis of MYOG polyclonal antibody
  • Western blot analysis of extracts of RD cells, using MYOG antibody at 1:1000 dilution.
  • Western blot analysis of extracts of RD cells, using MYOG antibody at 1:1000 dilution.
Product NameMYOG polyclonal antibody
Catalog No.BS8337
Swiss-ProtP15173
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IF
Application_all\"WB 1:500 - 1:2000 IF 1:50 - 1:200\"
BiowMW~ 25 kDa
Alternative NameMyogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4; MYOG; BHLHC3; MYF4
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
MYOG polyclonal antibody
Background :
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
MYOG polyclonal antibody detects endogenous levels of MYOG protein.
Immunogen :
Recombinant full length Human MYOG.
Conjugate :
Unconjugated
Modification :
Unmodification
  • WesternBlot (WB) analysis of MYOG polyclonal antibody
  • Western blot analysis of extracts of RD cells, using MYOG antibody at 1:1000 dilution.
  • Western blot analysis of extracts of RD cells, using MYOG antibody at 1:1000 dilution.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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