Product Name :
p38/MAPK14 (phospho-T180/Y182) polyclonal antibody Background :
MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p38/MAPK14 (phospho-Y182) polyclonal antibody detects endogenous levels of MAPK14 protein only when phosphorylated at Tyr182. Immunogen :
A synthetic phosphorylated peptide around Y182 of human MAPK14 (NP_620581.1). Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-Y182 pAb at 1:1000 dilution.
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  Western blot analysis of extracts of 3T3L1 cells, using Phospho-p38 MAPK-Y182 pAb at 1:1000 dilution.
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  Western blot analysis of extracts of 3T3L1 cells, using Phospho-p38 MAPK-Y182 pAb at 1:1000 dilution.
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  Western blot analysis of extracts of 3T3L1 cells, using Phospho-p38 MAPK-Y182 pAb at 1:1000 dilution.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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