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Tubulin α (G436) polyclonal antibody BS1699
  • Western blot (WB) analysis of Tubulin α (G436) pAb at 1:5000/1:10000/1:20000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:CT-26 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Tubulin α (G436) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of Tubulin α (G436) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameTubulin α (G436) polyclonal antibody
Catalog No.BS1699
Swiss-ProtQ71U36/P68363
Host Rabbit
ReactivityHuman,Mouse,Rat,Pig,Chicken,Odontobutis obscurus(not applicable to some tissue)
ApplicationsWB IHC IF
Application_allWB: 1:5000~20000 IHC: 1:100~500 IF: 1:500~1000
BiowMW~ 55 kDa
Alternative NameTubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1A; TUBA3; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $158
100ul $275
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Product Name :
Tubulin α (G436) polyclonal antibody
Background :
Tubulin is a major cytoskeleton component that has three distinct forms, designated α, β and γ Tubulin. α and β Tubulins form heterodimers, which multimerize to form a microtubule filament. γ Tubulin forms a soluble multiprotein particle with several other proteins. This particle, designated the gammasome, is required for nucleating microtubule filaments at the centrosome. In several organisms, numerous isoforms of the Tubulins exist that are encoded by different genes. The α and β isoforms undergo a variety of post-translational modifications, which may affect microtubule stability and protein interactions. High expression of class II β Tubulin has been seen in elongating axons, indicating a role in neurite outgrowth. Tubulins may also play a role in non-neuronal cell process formation.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Tubulin α (G436) polyclonal antibody detects endogenous levels of Tubulin α protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 201-256 of Human Tubulin α.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot (WB) analysis of Tubulin α (G436) pAb at 1:5000/1:10000/1:20000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:CT-26 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Tubulin α (G436) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of Tubulin α (G436) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
PMCID:    Pubmed No.: PMCID:    Pubmed No.: Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis PMCID:    Pubmed No.:28552346 Sex hormones establish a reserve pool of adult muscle stem cells PMCID:    Pubmed No.:28552346 Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis PMCID:    Pubmed No.:28552346 Sex hormones establish a reserve pool of adult muscle stem cells PMCID:    Pubmed No.:28552346 Coupling of mitochondrial function and skeletal muscle fiber type by a miR‐499/Fnip1/AMPK circuit PMCID:    Pubmed No.:27506764 miR-181b modulates multidrug resistance by targeting BCL2 in human cancer cell lines PMCID:    Pubmed No.:20162574 miR-497 modulates multidrug resistance of human cancer cell lines by targeting BCL2 PMCID:    Pubmed No.:21258880 miR-200bc/429 cluster modulates multidrug resistance of human cancer cell lines by targeting BCL2 and XIAP PMCID:    Pubmed No.:21993663 Does insulin bolster antioxidant defenses via the ERK-Akt-Nrf2 pathway ? 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PMCID:    Pubmed No.:24823476 Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer PMCID:    Pubmed No.:25546612 Blockage of Conformational Changes of Heat Shock Protein gp96 on Cell Membrane by a α-Helix Peptide Inhibits HER2 Dimerization and Signaling in Breast Cancer PMCID:    Pubmed No.:25898135 Effects of Ixeris dentata water extract and caffeic acid on allergic inflammation in vivo and in vitro PMCID:    Pubmed No.:26104582 RGS19 Converts Iron Deprivation Stress into a Growth-Inhibitory Signal PMCID:    Pubmed No.:26116529 Lipid-induced muscle insulin resistance is mediated by GGPPS via modulation of the RhoA/Rho-kinase signaling pathway. PMCID:    Pubmed No.:26112408 Dietary Fiber Intake is Associated with Increased Colonic Mucosal GPR43+ Polymorphonuclear Infiltration in Active Crohn's Disease PMCID:    Pubmed No.:26140540 p53-p66 shc/miR-21-Sod2 signaling is critical for the inhibitory effect of betulinic acid on hepatocellular carcinoma PMCID:    Pubmed No.:26222667 The Effects of HSP27 on Gemcitabine-Resistant Pancreatic Cancer Cell Line Through Snail PMCID:    Pubmed No.:26348464 Jumonji Domain Containing 5 (JMJD5) Associates with Spindle Microtubules and Is Required for Proper Mitosis PMCID:    Pubmed No.:26710852 PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells PMCID:    Pubmed No.:26860588 LSD1-mediated epigenetic modification contributes to ovarian cancer cell migration and invasion PMCID:    Pubmed No.:27109588 PP2A regulates kinetochore-microtubule attachment during meiosis I in oocyte PMCID:    Pubmed No.:27096707 Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer PMCID:    Pubmed No.:25546612 The Effects of HSP27 on Gemcitabine-Resistant Pancreatic Cancer Cell Line Through Snail PMCID:    Pubmed No.:26348464 Jumonji Domain Containing 5 (JMJD5) Associates with Spindle Microtubules and Is Required for Proper Mitosis PMCID:    Pubmed No.:26710852 PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells PMCID:    Pubmed No.:26860588 Enriched variations in TEKT4 and breast cancer resistance to paclitaxel. PMCID:    Pubmed No.:24823476 Neuroprotective Effects of Tetramethylpyrazine against Dopaminergic Neuron Injury in a Rat Model of Parkinson's Disease Induced by MPTP PMCID:    Pubmed No.:24719552 Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats PMCID:    Pubmed No.:29331496 Negative effects of long-term feeding of high-grain diets to lactating goats on milk fat production and composition by regulating gene expression and DNA methylation in the mammary gland PMCID:    Pubmed No.:29026537 Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle PMCID:    Pubmed No.:27738103 PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells PMCID:    Pubmed No.:26860588 Rhapontin ameliorates colonic epithelial dysfunction in experimental colitis through SIRT1 signaling PMCID:    Pubmed No.:27930969 Supplementing the maternal diet of rats with butyrate enhances mitochondrial biogenesis in the skeletal muscles of weaned offspring PMCID:    Pubmed No.:28091351 Selective activation of CB2 receptor improves efferocytosis in cultured macrophages PMCID:    Pubmed No.:27474129 Combined treatment with vitamin C and methotrexate inhibits triple-negative breast cancer cell growth by increasing H2O2 accumulation and activating caspase-3 and p38 pathways PMCID:    Pubmed No.:28259996 Heat Stress Reduces Sperm Motility via Activation of Glycogen Synthase Kinase-3α and Inhibition of Mitochondrial Protein Import PMCID:    Pubmed No.:29018353 Butyrate stimulates adipose lipolysis and mitochondrial oxidative phosphorylation through histone hyperacetylation‐associated β3‐adrenergic receptor activation in high‐fat diet‐induced obese mice PMCID:    Pubmed No.:28028849 CYR61 (CCN1) is a metastatic biomarker of gastric cardia adenocarcinoma PMCID:    Pubmed No.:27105510 Histone methyltransferase MLL4 controls myofiber identity and muscle performance through MEF2 interaction PMCID:    Pubmed No.:32544095 Sirtuin 6 is a negative regulator of FcεRI signaling and anaphylactic responses PMCID:    Pubmed No.:34051221 Reinforced-hydrogel encapsulated hMSCs towards brain injury treatment by trans-septal approach PMCID:    Pubmed No.:33038593 Exercise and dietary intervention ameliorate high-fat diet-induced NAFLD and liver aging by inducing lipophagy PMCID:    Pubmed No.:32863214 Magnesium protects against sepsis by blocking gasdermin D N-terminal-induced pyroptosis PMCID:    Pubmed No.:31209359 METTL3/METTL14 transactivation and m6A-dependent TGF-β1 translation in activated Kupffer cells PMCID:    Pubmed No.:33992834 Nogo-C regulates post myocardial infarction fibrosis through the interaction with ER Ca 2+ leakage channel Sec61α in mouse hearts PMCID:    Pubmed No.:29795235 AMPK-dependent and -independent coordination of mitochondrial function and muscle fiber type by FNIP1 PMCID:    Pubmed No.:33780446 Caspase‐1‐dependent mechanism mediating the harmful impacts of the quorum‐sensing molecule N‐(3‐oxo‐dodecanoyl)‐l‐homoserine lactone on the intestinal cells PMCID:    Pubmed No.:30471106 Inactivation of glycogen synthase kinase-3α is required for mitochondria-mediated apoptotic germ cell phagocytosis in Sertoli cells PMCID:    Pubmed No.:30398976 Myeloid cell-specific sirtuin 6 deficiency delays wound healing in mice by modulating inflammation and macrophage phenotypes PMCID:    Pubmed No.:31028245 NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving PMCID:    Pubmed No.:34088892 ASK1 inhibits proliferation and migration of lung cancer cells via inactivating TAZ PMCID:    Pubmed No.:33042617 CPI-17-mediated contraction of vascular smooth muscle is essential for the development of hypertension in obese mice PMCID:    Pubmed No.:30948334 Cancer‐associated fibroblasts promote PD‐L1 expression in mice cancer cells via secreting CXCL5 PMCID:    Pubmed No.:30873585 The amphioxus ERK2 gene is involved in innate immune response to LPS stimulation PMCID:    Pubmed No.:30439498 Abl-mediated PI3K activation regulates macrophage podosome formation PMCID:    Pubmed No.:32393599 NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β‐cells for protection PMCID:    Pubmed No.:33124187 Emodin prevents renal ischemia-reperfusion injury via suppression of CAMKII/DRP1-mediated mitochondrial fission PMCID:    Pubmed No.:34793771 Nitrosonisoldipine is a selective inhibitor of inflammatory caspases and protects against pyroptosis and related septic shock PMCID:    Pubmed No.:33454984 Dahuang Zhechong pill attenuates CCl4‐induced rat liver fibrosis via the PI3K‐Akt signaling pathway PMCID:    Pubmed No.:31502329 Sodium Butyrate Ameliorates High-Fat-Diet-Induced Non-alcoholic Fatty Liver Disease through Peroxisome Proliferator-Activated Receptor α-Mediated 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Pubmed No.:32772229 Endogenous peptide LYENRL prevents the activation of hypertrophic scar-derived fibroblasts by inhibiting the TGF-β1/Smad pathway PMCID:    Pubmed No.:31344427 Effects of chronic dexamethasone administration on hyperglycemia and insulin release in goats PMCID:    Pubmed No.:29568520 Anticancer effects of methotrexate in combination with α‑tocopherol and α‑tocopherol succinate on triple‑negative breast cancer PMCID:    Pubmed No.:30628707 HSC-specific knockdown of GGPPS alleviated CCl4-induced chronic liver fibrosis through mediating RhoA/Rock pathway PMCID:    Pubmed No.:31105844 CSNK1G2 differently sensitizes tamoxifen-induced decrease in PI3K/AKT/mTOR/S6K and ERK signaling according to the estrogen receptor existence in breast cancer cells PMCID:    Pubmed No.:33861751 Adipokine zinc-α2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the β3-AR/PKA/CREB pathway PMCID:    Pubmed No.:31260855 Antioxidant vitamins promote anticancer 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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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