Product Name :
MDFI (P134) polyclonal antibody Background :
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MDFI (P134) polyclonal antibody detects endogenous levels of MDFI protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-146 of Human MDFI. Conjugate :
Unconjugated Modification :
Unmodified

• 
  Western blot (WB) analysis of MDFI (P134) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug) Lane6:3T3-L1 whole cell lysate(40ug)
• 
  Immunofluorescence analysis of PC3 cells using MDFI (P134) pAb at dilution of 1:200.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---