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CD59 polyclonal antibody NCP0217P
  • Western blot (WB) analysis of CD59 polyclonal antibody at 1:1000 dilution Lane1:MCF-7 cell membrane lysate(25ug) Lane2:A549 cell membrane lysate(25ug) Lane3:L02 cell membrane lysate(25ug)
  • Immunofluorescence analysis of MCF-7 cells using CD59 pAb at dilution of 1:200.
Product NameCD59 polyclonal antibody
Catalog No.NCP0217P
Swiss-ProtP13987
Host Rabbit
ReactivityHuman
ApplicationsWB IF
Application_allWB: 1:1000~1:2000 IF 1:50~1:200
BiowMW~17 kDa
Alternative NameCD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; CD59
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $158
100ul $275
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Product Name :
CD59 polyclonal antibody
Background :
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
CD59 polyclonal antibody detects endogenous levels of CD59 protein.
Immunogen :
Recombinant protein of human CD59.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot (WB) analysis of CD59 polyclonal antibody at 1:1000 dilution Lane1:MCF-7 cell membrane lysate(25ug) Lane2:A549 cell membrane lysate(25ug) Lane3:L02 cell membrane lysate(25ug)
  • Immunofluorescence analysis of MCF-7 cells using CD59 pAb at dilution of 1:200.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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