Product Name :
CD9 polyclonal antibody Background :
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion.Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity).In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity).In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles.Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity).Acts as a receptor for PSG17 (By similarity).Involved in platelet activation and aggregation.Regulates paranodal junction formation (By similarity).Involved in cell adhesion, cell motility and tumor metastasis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CD9 polyclonal antibody detects endogenous levels of CD9 protein. Immunogen :
Recombinant protein of human CD9. Conjugate :
Unconjugated Modification :
Unmodified

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  Western blot (WB) analysis of CD9 polyclonal antibody at 1:1000 dilution Lane1:EC9706 whole cell lysate(30ug) Lane2:L02 whole cell lysate(30ug) Lane3:A549 whole cell lysate(30ug) Lane4:MCF-7 whole cell lysate(30ug)
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  Immunofluorescence analysis of L02 cells using CD9 pAb at dilution of 1:200.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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