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TLR4 (S441) polyclonal antibody BS3489
  • Western blot (WB) analysis of TLR4 (S441) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:Myla2059 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate (40ug)
  • Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue.
  • Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue.
Product NameTLR4 (S441) polyclonal antibody
Catalog No.BS3489
Swiss-ProtO00206
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF IP
Application_allWB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
BiowMW~ 120 kDa
Alternative NameToll-like receptor 4; hToll; CD284; TLR-4
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $158
100ul $275
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Product Name :
TLR4 (S441) polyclonal antibody
Background :
Toll like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti fungal response, while the related 18 wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram negative bacteria.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
TLR4 (S441) polyclonal antibody detects endogenous levels of TLR4 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 421-470 of Human TLR4.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot (WB) analysis of TLR4 (S441) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:Myla2059 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate (40ug)
  • Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue.
  • Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue.
Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma PMCID:    Pubmed No.:25511737 Peroxisome proliferator-activated receptor gamma agonist rosiglitazone attenuates oxyhemoglobin-induced Toll-like receptor 4 expression in vascular smooth muscle cells PMCID:    Pubmed No.:20132800 Therapeutic potential of peroxisome proliferator-activated receptor gamma agonist rosiglitazone in cerebral vasospasm after a rat experimental subarachnoid hemorrhage model PMCID:    Pubmed No.:21440907 Schisandrin B exerts anti-neuroinflammatory activity by inhibiting the Toll-like receptor 4-dependent MyD88/IKK/NF-κB signaling pathway in lipopolysaccharide-induced microglia PMCID:    Pubmed No.:22698579 Toll-like receptor 4 signaling promotes epithelial-mesenchymal transition in human hepatocellular carcinoma induced by lipopolysaccharide PMCID:    Pubmed No.:22938142 Protosappanin A inhibits oxidative and nitrative stress via interfering the interaction of transmembrane protein CD14 with Toll-like receptor-4 in 3 lipopolysaccharide-induced BV-2 microglia PMCID:    Pubmed No.:23000519 Systemic cytokine profiles and splenic toll-like receptor expression during Trichinella spiralis infection PMCID:    Pubmed No.:23466989 Prospective study of the safety and efficacy of intermittent inflow occlusion (Pringle maneuver) in living donor left hepatectomy PMCID:    Pubmed No.:25220784 Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma PMCID:    Pubmed No.:25511737 Involvement of TLR2 and TLR9 in the anti-inflammatory effects of chlorogenic acid in HSV-1-infected microglia PMCID:    Pubmed No.:25744394 Protective effects of protocatechuic acid on acute lung injury induced by lipopolysaccharide in mice via p38MAPK and NF-κB signal pathways PMCID:    Pubmed No.:25841318 Cinnamaldehyde inhibits inflammation and brain damage in a mouse model of permanent cerebral ischemia. PMCID:    Pubmed No.:26234631 TLR3 and TLR4 as potential clinical biomarkers for in-stent restenosis in drug-eluting stents patients PMCID:    Pubmed No.:26318748 Aging increases the susceptibility of cisplatin-induced nephrotoxicity PMCID:    Pubmed No.:26534724 Changes in blood monocyte Toll-like receptor (TLR)2/4 and serum surfactant protein A (SP-A) reveal a pathophysiological mechanism for community-acquired pneumonia in patients with Type 2 diabetes. PMCID:    Pubmed No.:26648341 Increased expression of RP105 decreases cardiomyocytes hypoxia/reoxygenation injury through the TLR4/NF-κB signaling pathway PMCID:    Pubmed No.:25187802 Sinomenine down-regulates TLR4/TRAF6 expression and attenuates lipopolysaccharide-induced osteoclastogenesis and osteolysis PMCID:    Pubmed No.:26965104 Prospective study of the safety and efficacy of intermittent inflow occlusion (Pringle maneuver) in living donor left hepatectomy PMCID:    Pubmed No.:25220784 Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma PMCID:    Pubmed No.:25511737 Aging increases the susceptibility of cisplatin-induced nephrotoxicity PMCID:    Pubmed No.:26534724 Changes in blood monocyte Toll-like receptor (TLR)2/4 and serum surfactant protein A (SP-A) reveal a pathophysiological mechanism for community-acquired pneumonia in patients with Type 2 diabetes. PMCID:    Pubmed No.:26648341 Increased expression of RP105 decreases cardiomyocytes hypoxia/reoxygenation injury through the TLR4/NF-κB signaling pathway PMCID:    Pubmed No.:25187802 Sinomenine down-regulates TLR4/TRAF6 expression and attenuates lipopolysaccharide-induced osteoclastogenesis and osteolysis PMCID:    Pubmed No.:26965104 The C-Terminal Disulfide Bonds of Helicobacter pylori GroES Are Critical for IL-8 Secretion via the TLR4-Dependent Pathway in Gastric Epithelial Cells PMCID:    Pubmed No.:25769921 The mAb against adipocyte fatty acid-binding protein 2E4 attenuates the inflammation in the mouse model of high-fat diet-induced obesity via toll-like receptor 4 pathway PMCID:    Pubmed No.:25596549 Sinomenine down-regulates TLR4/TRAF6 expression and attenuates lipopolysaccharide-induced osteoclastogenesis and osteolysis PMCID:    Pubmed No.:26965104 TLR3 and TLR4 as potential clinical biomarkers for in-stent restenosis in drug-eluting stents patients PMCID:    Pubmed No.:26318748 Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms PMCID:    Pubmed No.:28011393 Foxo1-mediated inflammatory response after cerebral hemorrhage in rats PMCID:    Pubmed No.:27288017 Changes in blood monocyte Toll‐like receptor and serum surfactant protein A reveal a pathophysiological mechanism for community‐acquired pneumonia in patients with type 2 diabetes PMCID:    Pubmed No.:26648341 Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks PMCID:    Pubmed No.:34022569 Salvianolic acid A alleviated inflammatory response mediated by microglia through inhibiting the activation of TLR2/4 in acute cerebral ischemia-reperfusion PMCID:    Pubmed No.:33985878 Curcumin improves memory deficits by inhibiting HMGB1-RAGE/TLR4-NF-κB signalling pathway in APPswe/PS1dE9 transgenic mice hippocampus PMCID:    Pubmed No.:34405526 MyD88 determines the protective effects of fish oil and perilla oil against metabolic disorders and inflammation in adipose tissue from mice fed a high-fat diet PMCID:    Pubmed No.:34168108 Ulinastatin mediates suppression of regulatory T cells through TLR4/NF-κB signaling pathway in murine sepsis PMCID:    Pubmed No.:30261464 Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization PMCID:    Pubmed No.:29753749 Role of TREM-1 in the development of early brain injury after subarachnoid hemorrhage PMCID:    Pubmed No.:33727099 Mas-related G protein-coupled receptor D participates in inflammatory pain by promoting NF-κB activation through interaction with TAK1 and IKK complex PMCID:    Pubmed No.:33080316 Ethyl pyruvate enhances spontaneous remyelination by targeting microglia phagocytosis PMCID:    Pubmed No.:31677497 Dynamic Balance of Microglia and Astrocytes Involved in the Remyelinating Effect of Ginkgolide B PMCID:    Pubmed No.:31969806 Melanin of Sporothrix globosa affects the function of THP-1 macrophages and modulates the expression of TLR2 and TLR4 PMCID:    Pubmed No.:34454025 Evidence of anti-inflammatory activity of Schizandrin A in animal models of acute inflammation PMCID:    Pubmed No.:32076762 Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-κB pathway PMCID:    Pubmed No.:32915230 Toll-Like Receptors 2 and 4 Predict New-Onset Atrial Fibrillation in Acute Myocardial Infarction Patients PMCID:    Pubmed No.:29375116 Stellate ganglion block reduces inflammation and improves neurological function in diabetic rats during ischemic stroke PMCID:    Pubmed No.:35142688 Molecular Hydrogen Mediates Neurorestorative Effects After Stroke in Diabetic Rats: the TLR4/NF-κB Inflammatory Pathway PMCID:    Pubmed No.:35895245 Oxidative Stress and Pyroptosis in Doxorubicin-Induced Heart Failure and Atrial Fibrillation PMCID:    Pubmed No.:36733418 Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages PMCID:    Pubmed No.:37141335 TLR4 inhibited autophagy by modulating PI3K/AKT/mTOR signaling pathway in Gastric cancer cell lines PMCID:    Pubmed No.:37257791 PAG neuronal NMDARs activation mediated morphine-induced hyperalgesia by HMGB1-TLR4 dependent microglial inflammation PMCID:    Pubmed No.:37352811 Levomilnacipran ameliorates lipopolysaccharide-induced depression-like behaviors and suppressed the TLR4/Ras signaling pathway PMCID:    Pubmed No.:37413934
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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