Product Name :
TLR4 (S441) polyclonal antibody Background :
Toll like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti fungal response, while the related 18 wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram negative bacteria. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TLR4 (S441) polyclonal antibody detects endogenous levels of TLR4 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 421-470 of Human TLR4. Conjugate :
Unconjugated Modification :
Unmodified
TLR4 (S441) polyclonal antibody Background :
Toll like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti fungal response, while the related 18 wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram negative bacteria. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TLR4 (S441) polyclonal antibody detects endogenous levels of TLR4 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 421-470 of Human TLR4. Conjugate :
Unconjugated Modification :
Unmodified
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Western blot (WB) analysis of TLR4 (S441) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:Myla2059 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate (40ug)
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Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue.
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Immunohistochemistry (IHC) analyzes of TLR4 (S441) pAb in paraffin-embedded human brain tissue.
Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma
PMCID: Pubmed No.:25511737
Peroxisome proliferator-activated receptor gamma agonist rosiglitazone attenuates oxyhemoglobin-induced Toll-like receptor 4 expression in vascular smooth muscle cells
PMCID: Pubmed No.:20132800
Therapeutic potential of peroxisome proliferator-activated receptor gamma agonist rosiglitazone in cerebral vasospasm after a rat experimental subarachnoid hemorrhage model
PMCID: Pubmed No.:21440907
Schisandrin B exerts anti-neuroinflammatory activity by inhibiting the Toll-like receptor 4-dependent MyD88/IKK/NF-κB signaling pathway in lipopolysaccharide-induced microglia
PMCID: Pubmed No.:22698579
Toll-like receptor 4 signaling promotes epithelial-mesenchymal transition in human hepatocellular carcinoma induced by lipopolysaccharide
PMCID: Pubmed No.:22938142
Protosappanin A inhibits oxidative and nitrative stress via interfering the interaction of transmembrane protein CD14 with Toll-like receptor-4 in 3 lipopolysaccharide-induced BV-2 microglia
PMCID: Pubmed No.:23000519
Systemic cytokine profiles and splenic toll-like receptor expression during Trichinella spiralis infection
PMCID: Pubmed No.:23466989
Prospective study of the safety and efficacy of intermittent inflow occlusion (Pringle maneuver) in living donor left hepatectomy
PMCID: Pubmed No.:25220784
Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma
PMCID: Pubmed No.:25511737
Involvement of TLR2 and TLR9 in the anti-inflammatory effects of chlorogenic acid in HSV-1-infected microglia
PMCID: Pubmed No.:25744394
Protective effects of protocatechuic acid on acute lung injury induced by lipopolysaccharide in mice via p38MAPK and NF-κB signal pathways
PMCID: Pubmed No.:25841318
Cinnamaldehyde inhibits inflammation and brain damage in a mouse model of permanent cerebral ischemia.
PMCID: Pubmed No.:26234631
TLR3 and TLR4 as potential clinical biomarkers for in-stent restenosis in drug-eluting stents patients
PMCID: Pubmed No.:26318748
Aging increases the susceptibility of cisplatin-induced nephrotoxicity
PMCID: Pubmed No.:26534724
Changes in blood monocyte Toll-like receptor (TLR)2/4 and serum surfactant protein A (SP-A) reveal a pathophysiological mechanism for community-acquired pneumonia in patients with Type 2 diabetes.
PMCID: Pubmed No.:26648341
Increased expression of RP105 decreases cardiomyocytes hypoxia/reoxygenation injury through the TLR4/NF-κB signaling pathway
PMCID: Pubmed No.:25187802
Sinomenine down-regulates TLR4/TRAF6 expression and attenuates lipopolysaccharide-induced osteoclastogenesis and osteolysis
PMCID: Pubmed No.:26965104
Prospective study of the safety and efficacy of intermittent inflow occlusion (Pringle maneuver) in living donor left hepatectomy
PMCID: Pubmed No.:25220784
Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma
PMCID: Pubmed No.:25511737
Aging increases the susceptibility of cisplatin-induced nephrotoxicity
PMCID: Pubmed No.:26534724
Changes in blood monocyte Toll-like receptor (TLR)2/4 and serum surfactant protein A (SP-A) reveal a pathophysiological mechanism for community-acquired pneumonia in patients with Type 2 diabetes.
PMCID: Pubmed No.:26648341
Increased expression of RP105 decreases cardiomyocytes hypoxia/reoxygenation injury through the TLR4/NF-κB signaling pathway
PMCID: Pubmed No.:25187802
Sinomenine down-regulates TLR4/TRAF6 expression and attenuates lipopolysaccharide-induced osteoclastogenesis and osteolysis
PMCID: Pubmed No.:26965104
The C-Terminal Disulfide Bonds of Helicobacter pylori GroES Are Critical for IL-8 Secretion via the TLR4-Dependent Pathway in Gastric Epithelial Cells
PMCID: Pubmed No.:25769921
The mAb against adipocyte fatty acid-binding protein 2E4 attenuates the inflammation in the mouse model of high-fat diet-induced obesity via toll-like receptor 4 pathway
PMCID: Pubmed No.:25596549
Sinomenine down-regulates TLR4/TRAF6 expression and attenuates lipopolysaccharide-induced osteoclastogenesis and osteolysis
PMCID: Pubmed No.:26965104
TLR3 and TLR4 as potential clinical biomarkers for in-stent restenosis in drug-eluting stents patients
PMCID: Pubmed No.:26318748
Astragaloside IV attenuates cognitive impairments induced by transient cerebral ischemia and reperfusion in mice via anti-inflammatory mechanisms
PMCID: Pubmed No.:28011393
Foxo1-mediated inflammatory response after cerebral hemorrhage in rats
PMCID: Pubmed No.:27288017
Changes in blood monocyte Toll‐like receptor and serum surfactant protein A reveal a pathophysiological mechanism for community‐acquired pneumonia in patients with type 2 diabetes
PMCID: Pubmed No.:26648341
Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks
PMCID: Pubmed No.:34022569
Salvianolic acid A alleviated inflammatory response mediated by microglia through inhibiting the activation of TLR2/4 in acute cerebral ischemia-reperfusion
PMCID: Pubmed No.:33985878
Curcumin improves memory deficits by inhibiting HMGB1-RAGE/TLR4-NF-κB signalling pathway in APPswe/PS1dE9 transgenic mice hippocampus
PMCID: Pubmed No.:34405526
MyD88 determines the protective effects of fish oil and perilla oil against metabolic disorders and inflammation in adipose tissue from mice fed a high-fat diet
PMCID: Pubmed No.:34168108
Ulinastatin mediates suppression of regulatory T cells through TLR4/NF-κB signaling pathway in murine sepsis
PMCID: Pubmed No.:30261464
Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization
PMCID: Pubmed No.:29753749
Role of TREM-1 in the development of early brain injury after subarachnoid hemorrhage
PMCID: Pubmed No.:33727099
Mas-related G protein-coupled receptor D participates in inflammatory pain by promoting NF-κB activation through interaction with TAK1 and IKK complex
PMCID: Pubmed No.:33080316
Ethyl pyruvate enhances spontaneous remyelination by targeting microglia phagocytosis
PMCID: Pubmed No.:31677497
Dynamic Balance of Microglia and Astrocytes Involved in the Remyelinating Effect of Ginkgolide B
PMCID: Pubmed No.:31969806
Melanin of Sporothrix globosa affects the function of THP-1 macrophages and modulates the expression of TLR2 and TLR4
PMCID: Pubmed No.:34454025
Evidence of anti-inflammatory activity of Schizandrin A in animal models of acute inflammation
PMCID: Pubmed No.:32076762
Emodin protects against intestinal and lung injury induced by acute intestinal injury by modulating SP-A and TLR4/NF-κB pathway
PMCID: Pubmed No.:32915230
Toll-Like Receptors 2 and 4 Predict New-Onset Atrial Fibrillation in Acute Myocardial Infarction Patients
PMCID: Pubmed No.:29375116
Stellate ganglion block reduces inflammation and improves neurological function in diabetic rats during ischemic stroke
PMCID: Pubmed No.:35142688
Molecular Hydrogen Mediates Neurorestorative Effects After Stroke in Diabetic Rats: the TLR4/NF-κB Inflammatory Pathway
PMCID: Pubmed No.:35895245
Oxidative Stress and Pyroptosis in Doxorubicin-Induced Heart Failure and Atrial Fibrillation
PMCID: Pubmed No.:36733418
Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages
PMCID: Pubmed No.:37141335
TLR4 inhibited autophagy by modulating PI3K/AKT/mTOR signaling pathway in Gastric cancer cell lines
PMCID: Pubmed No.:37257791
PAG neuronal NMDARs activation mediated morphine-induced hyperalgesia by HMGB1-TLR4 dependent microglial inflammation
PMCID: Pubmed No.:37352811
Levomilnacipran ameliorates lipopolysaccharide-induced depression-like behaviors and suppressed the TLR4/Ras signaling pathway
PMCID: Pubmed No.:37413934
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.