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hptII (2D3) monoclonal antibody MB3065
  • Western blot analysis of HptII in Trangenic rice and Nontrangenic rice lysates using HptII antibody.Betaactin antibody was used as a loading control.
Product NamehptII (2D3) monoclonal antibody
Catalog No.MB3065
Swiss-Prot-
Host Mouse
ReactivityTransfected
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 37 kDa; Observed MW: 37 kDa
Alternative Name-
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
hptII (2D3) monoclonal antibody
Background :
Hygromycin is a commonly used for resistance screening. hptII is a protein kinase, which inactivates hygromycin by phosphorylating.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG2a
Immunogen :
A synthesized peptide derived from hptII
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of HptII in Trangenic rice and Nontrangenic rice lysates using HptII antibody.Betaactin antibody was used as a loading control.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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