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Wnt3 (4F11) monoclonal antibody MB3086
  • Western blot analysis of Wnt3 in CHO-K1 lysates and CHO-K1 transfected by Wnt3 lysates using Wnt3 antibody.
Product NameWnt3 (4F11) monoclonal antibody
Catalog No.MB3086
Swiss-ProtP56703
Host Mouse
ReactivityTransfected
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 40 kDa; Observed MW: 40 kDa
Alternative NameProto-oncogene Wnt-3; INT4; TETAMS
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Wnt3 (4F11) monoclonal antibody
Background :
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Purified recombinant human Wnt3 protein fragments expressed in E.coli.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Wnt3 in CHO-K1 lysates and CHO-K1 transfected by Wnt3 lysates using Wnt3 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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