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E Cadherin (6B10) monoclonal antibody MB3101
  • Western blot analysis of ECadherin in T47D, HCT116, MDA-MB-468 and Caco2 lysates using ECadherin antibody.
  • Western blot analysis of E Cadherin in mouse embryos and HCT116 lysates using ECadherin antibody.
  • Western blot analysis of E Cadherin in mouse embryos and HCT116 lysates using ECadherin antibody.
Product NameE Cadherin (6B10) monoclonal antibody
Catalog No.MB3101
Swiss-ProtP12830
Host Mouse
ReactivityHuman,Mouse
ApplicationsWB,IHC-P
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100
BiowMWCalculated MW: 97 kDa; Observed MW: 135 kDa
Alternative NameCDH1; CDHE; UVO; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD antigen CD324
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
E Cadherin (6B10) monoclonal antibody
Background :
CDH1 a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
A synthesized peptide derived from human E Cadherin
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of ECadherin in T47D, HCT116, MDA-MB-468 and Caco2 lysates using ECadherin antibody.
  • Western blot analysis of E Cadherin in mouse embryos and HCT116 lysates using ECadherin antibody.
  • Western blot analysis of E Cadherin in mouse embryos and HCT116 lysates using ECadherin antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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