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beta Catenin (5D6) monoclonal antibody MB3115
  • Western blot analysis of βCatenin in HCT116, C6 and 3T3 lysates using βCatenin antibody.
Product Namebeta Catenin (5D6) monoclonal antibody
Catalog No.MB3115
Swiss-ProtP35222
Host Mouse
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 85 kDa; Observed MW: 92 kDa
Alternative NameCTNNB1; CTNNB; OK/SW-cl.35; Catenin beta-1; Beta-catenin
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
beta Catenin (5D6) monoclonal antibody
Background :
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Purified recombinant human β-Catenin protein fragments expressed in E.coli.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of βCatenin in HCT116, C6 and 3T3 lysates using βCatenin antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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