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Prostate Specific Antigen monoclonal antibody MB3158
  • Western blot analysis of Prostate Specific Antigen in Human KLK3 using Prostate Specific Antigen antibody.
  • Flow Cytometry analysis of HeLa cells stained with KLK3 antibody and negative control .
Product NameProstate Specific Antigen monoclonal antibody
Catalog No.MB3158
Swiss-ProtP07288
Host Mouse
ReactivityHuman
ApplicationsWB,FC
Application_allWB: 1/500-1/1000 FC: 1/50-1/100
BiowMWCalculated MW: 29 kDa; Observed MW: 37 kDa
Alternative NameKLK3; APS; Prostate-specific antigen; PSA; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Prostate Specific Antigen monoclonal antibody
Background :
PSA/KLK3 is a member of the glandular kallikrein subfamily of serine proteases. Upon cleavage of 7 amino-terminal amino acids, it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Purified recombinant fragment of human KLK3 expressed in E. Coli.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Prostate Specific Antigen in Human KLK3 using Prostate Specific Antigen antibody.
  • Flow Cytometry analysis of HeLa cells stained with KLK3 antibody and negative control .
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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