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GFAP (9A2) monoclonal antibody MB3176
  • Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GFAP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of GFAP in mouse brain tissue using GFAP antibody,and DAPI .
Product NameGFAP (9A2) monoclonal antibody
Catalog No.MB3176
Swiss-ProtP14136
Host Mouse
ReactivityHuman,Mouse
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 50 kDa; Observed MW: 50 kDa
Alternative NameGFAP; FLJ45472; cb345; ALXDRD
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
GFAP (9A2) monoclonal antibody
Background :
GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Synthetic Peptide of GFAP
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GFAP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of GFAP in mouse brain tissue using GFAP antibody,and DAPI .
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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