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ABCB5 (8D2) monoclonal antibody MB3183
  • Western blot analysis of ABCB5 in K562 lysates using ABCB5 antibody.
  • Western blot analysis of ABCB5 in HepG2, K562 lysates using ABCB5 antibody.
  • Western blot analysis of ABCB5 in HepG2, K562 lysates using ABCB5 antibody.
Product NameABCB5 (8D2) monoclonal antibody
Catalog No.MB3183
Swiss-ProtQ2M3G0
Host Mouse
ReactivityHuman
ApplicationsWB,ICC/IF
Application_allWB: 1/500-1/1000 IF: 1/50-1/200
BiowMWCalculated MW: 139 kDa; Observed MW: 90 kDa
Alternative NameABCB5 P-gp; ATP binding cassette sub family B (MDR/TAP) member 5; P glycoprotein ABCB5
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
ABCB5 (8D2) monoclonal antibody
Background :
Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Synthetic Peptide of ABCB5
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of ABCB5 in K562 lysates using ABCB5 antibody.
  • Western blot analysis of ABCB5 in HepG2, K562 lysates using ABCB5 antibody.
  • Western blot analysis of ABCB5 in HepG2, K562 lysates using ABCB5 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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