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Cytokeratin 6 (1A5) monoclonal antibody MB3184
  • Immunohistochemistry analysis of paraffin-embedded Human breastcancer tissue using Cytokeratin 6 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Cytokeratin 6 in Human breastcancer tissue using Cytokeratin 6 antibody,and DAPI .
  • Immunofluorescence analysis of Cytokeratin 6 in Human breastcancer tissue using Cytokeratin 6 antibody,and DAPI .
Product NameCytokeratin 6 (1A5) monoclonal antibody
Catalog No.MB3184
Swiss-ProtP02538/P04259/P48668
Host Mouse
ReactivityHuman
ApplicationsWB,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 60 kDa; Observed MW: 56-60 kDa
Alternative NameCK 6A; CK 6B; CK 6C; CK 6D; CK 6E; CK-6C; CK-6E; K6a keratin; K6b keratin; K6C; K6c keratin; K6d keratin; K6e keratin; Keratin; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
Cytokeratin 6 (1A5) monoclonal antibody
Background :
There are at least six isoforms of human type II keratin-6 (K6). There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa, respectively).
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Synthetic Peptide of Cytokeratin 6
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunohistochemistry analysis of paraffin-embedded Human breastcancer tissue using Cytokeratin 6 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of Cytokeratin 6 in Human breastcancer tissue using Cytokeratin 6 antibody,and DAPI .
  • Immunofluorescence analysis of Cytokeratin 6 in Human breastcancer tissue using Cytokeratin 6 antibody,and DAPI .
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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