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beta III Tubulin (3H2) monoclonal antibody MB3214
  • Western blot analysis of beta III Tubulin in various cell lines using β3Tubulin antibody.
  • Western blot analysis of beta III Tubulin in mouse brain, rat brain lysates using beta III Tubulin antibody.
Product Namebeta III Tubulin (3H2) monoclonal antibody
Catalog No.MB3214
Swiss-ProtQ13509
Host Mouse
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1/500-1/1000
BiowMWCalculated MW: 50 kDa; Observed MW: 50 kDa
Alternative NameTUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
beta III Tubulin (3H2) monoclonal antibody
Background :
TUBB3 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Synthetic Peptide of β III tubulin
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of beta III Tubulin in various cell lines using β3Tubulin antibody.
  • Western blot analysis of beta III Tubulin in mouse brain, rat brain lysates using beta III Tubulin antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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