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EGFR (3A9) monoclonal antibody MB3226
  • Immunofluorescence analysis of EGFR in Hela using EGFR antibody and DAPI .
  • Western blot analysis of EGFR in Hela lysates using EGFR antibody
  • Western blot analysis of EGFR in Hela lysates using EGFR antibody
  • Western blot analysis of EGFR in Hela lysates using EGFR antibody
Product NameEGFR (3A9) monoclonal antibody
Catalog No.MB3226
Swiss-ProtP00533
Host Mouse
ReactivityHuman
ApplicationsWB,IHC-F,IHC-P,ICC/IF
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
BiowMWCalculated MW: 134 kDa; Observed MW: 170 kDa
Alternative NameEGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $258
100ul $398
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Product Name :
EGFR (3A9) monoclonal antibody
Background :
EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG1
Immunogen :
Synthetic Peptide of EGFR
Conjugate :
Unconjugated
Modification :
Unmodified
  • Immunofluorescence analysis of EGFR in Hela using EGFR antibody and DAPI .
  • Western blot analysis of EGFR in Hela lysates using EGFR antibody
  • Western blot analysis of EGFR in Hela lysates using EGFR antibody
  • Western blot analysis of EGFR in Hela lysates using EGFR antibody
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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