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CD133 polyclonal antibody BZ16196
  • Western blot analysis of CD133 in A549, SW620, HT-29, Jurkat, mouse kidney, mouse lung lysates using CD133 antibody.
Product NameCD133 polyclonal antibody
Catalog No.BZ16196
Swiss-ProtO43490
Host Rabbit
ReactivityHuman,Mouse
ApplicationsWB,IHC-P,ELISA
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
BiowMWCalculated MW: 97 kDa; Observed MW: 100 kDa
Alternative NameProminin-1; Antigen AC133; Prominin-like protein 1; CD133.
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
CD133 polyclonal antibody
Background :
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
The antiserum was produced against synthesized peptide derived from the N-terminal region of human PROM1. AA range:41-90
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of CD133 in A549, SW620, HT-29, Jurkat, mouse kidney, mouse lung lysates using CD133 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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