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ASAH3 polyclonal antibody BZ16221
  • Western blot analysis of ASAH3 in HepG2 lysates using ASAH3 antibody.
Product NameASAH3 polyclonal antibody
Catalog No.BZ16221
Swiss-ProtQ8TDN7
Host Rabbit
ReactivityHuman
ApplicationsWB,ELISA
Application_allWB: 1/500-1/1000 ELISA: 1/10000
BiowMWCalculated MW: 31 kDa; Observed MW: 34 kDa
Alternative NameACER1; ASAH3; Alkaline ceramidase 1; AlkCDase 1; Alkaline CDase 1; Acylsphingosine deacylase 3; N-acylsphingosine amidohydrolase 3
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
ASAH3 polyclonal antibody
Background :
Endoplasmic reticulum ceramidase that catalyzes the hydrolysis of ceramides into sphingosine and free fatty acids at alkaline pH.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Synthesized peptide derived from ASAH3 . at AA range: 100-180
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of ASAH3 in HepG2 lysates using ASAH3 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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