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Collagen IV alpha 1 polyclonal antibody BZ16229
  • Western blot analysis of Collagen IV alpha 1 in mouse brain lysates using Collagen IV alpha 1 antibody.
Product NameCollagen IV alpha 1 polyclonal antibody
Catalog No.BZ16229
Swiss-ProtP02462
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,ELISA
Application_allWB: 1/500-1/1000 ELISA: 1/10000
BiowMWCalculated MW: 161 kDa; Observed MW: 130 kDa
Alternative NameCollagen alpha-1(IV) chain [Cleaved into: Arresten]
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Collagen IV alpha 1 polyclonal antibody
Background :
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
Synthesized peptide derived from Collagen IV . at AA range: 1620-1670
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Collagen IV alpha 1 in mouse brain lysates using Collagen IV alpha 1 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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