Product Name :
Phospho-alpha 1 Sodium Potassium ATPase (Ser23) polyclonal antibody Background :
The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IgG Immunogen :
The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64 Conjugate :
Unconjugated Modification :
Phosphorylated

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  Western blot analysis of Phospho-alpha 1 Sodium Potassium ATPase in rat lysates using Phospho-alpha 1 Sodium Potassium ATPase antibody.
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  Immunofluorescence analysis of Phospho-alpha 1 Sodium Potassium ATPase in NIH/3T3 using ATP1 alpha1/Na+K+ ATPase1 antibody. The picture on the right is blocked using the Phospho- peptide.
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  Immunofluorescence analysis of Phospho-alpha 1 Sodium Potassium ATPase in NIH/3T3 using ATP1 alpha1/Na+K+ ATPase1 antibody. The picture on the right is blocked using the Phospho- peptide.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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