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Collagen I alpha 2 polyclonal antibody BZ16434
  • Western blot analysis of Collagen I alpha 2 in 823, 22RV293UV lysates using Collagen I alpha 2 antibody.
Product NameCollagen I alpha 2 polyclonal antibody
Catalog No.BZ16434
Swiss-ProtP08123
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-F,IHC-P,ICC/IF,ELISA
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
BiowMWCalculated MW: 129 kDa; Observed MW: 200 kDa
Alternative NameCollagen alpha-2(I) chain (Alpha-2 type I collagen)
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Collagen I alpha 2 polyclonal antibody
Background :
This gene encodes one of the chains for type I collagen, the fibrillar collagen found in most connective tissues. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
The antiserum was produced against synthesized peptide derived from human Collagen I. AA range:1-50
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Collagen I alpha 2 in 823, 22RV293UV lysates using Collagen I alpha 2 antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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