Product Name :
Phospho-IKK beta (Tyr188) polyclonal antibody Background :
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IgG Immunogen :
The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr188. AA range:161-210 Conjugate :
Unconjugated Modification :
Phosphorylated

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  Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-IKK beta antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right.
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  EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide , using IKKbeta (Phospho-Tyr18antibody
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  EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide , using IKKbeta (Phospho-Tyr18antibody
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  EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide , using IKKbeta (Phospho-Tyr18antibody

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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