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Rad51 polyclonal antibody BZ16550
  • Western blot analysis of Rad51 in HEK293 lysates; K562 lysates using Rad51 antibody.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Rad51 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Product NameRad51 polyclonal antibody
Catalog No.BZ16550
Swiss-ProtQ06609
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-P,ICC/IF,IP,FC
Application_allWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
BiowMWCalculated MW: 37 kDa; Observed MW: 37 kDa
Alternative NameRAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Purification&PurityAffinity Purified
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
Rad51 polyclonal antibody
Background :
Rad51 participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.
Product :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
IgG
Immunogen :
A synthesized peptide derived from human Rad51
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of Rad51 in HEK293 lysates; K562 lysates using Rad51 antibody.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Rad51 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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