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BAG3 polyclonal antibody BS75975
  • Western blot analysis of extracts of various cell lines, using BAG3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product NameBAG3 polyclonal antibody
Catalog No.BS75975
Swiss-ProtO95817
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF/ICC, IP
Application_allWB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100|IP,1:50 - 1:100
BiowMW80kDa
Alternative NameBAG3;BAG-3;BIS;CAIR-1;MFM6
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
BAG3 polyclonal antibody
Background :
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Unmodification
Immunogen :
Recombinant fusion protein of human BAG3(NP_004272.2).
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of various cell lines, using BAG3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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