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Phospho-PLC gamma 2 (PLCG2)-Y1217 polyclonal antibody BS79434
  • Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 -Y1217 antibody at 1:1000 dilution or PLC gamma 2 antibody . Raji cells were treated by Pervanadate at 37℃ for 30 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit .
    Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-PLC gamma 2 -Y1217 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product NamePhospho-PLC gamma 2 (PLCG2)-Y1217 polyclonal antibody
Catalog No.BS79434
Swiss-ProtP16885
Host Rabbit
ReactivityHuman, Mouse
ApplicationsWB, IHC
Application_allWB,1:500 - 1:2000|IHC,1:50 - 1:200
BiowMW150kDa
Alternative NamePLCG2;APLAID;FCAS3;PLC-IV;PLC-gamma-2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylated
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Size Price
50ul $258
100ul $398
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Product Name :
Phospho-PLC gamma 2 (PLCG2)-Y1217 polyclonal antibody
Background :
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Phosphorylated
Immunogen :
A synthetic phosphorylated peptide around Y1217 of human PLC gamma 2 (PLCG2)(NP_002652.2).
Conjugate :
Unconjugated
Modification :
Phosphorylated
  • Western blot analysis of extracts of Raji cells, using Phospho-PLC gamma 2 -Y1217 antibody at 1:1000 dilution or PLC gamma 2 antibody . Raji cells were treated by Pervanadate at 37℃ for 30 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit .
    Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-PLC gamma 2 -Y1217 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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