CYLD polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CYLD polyclonal antibody Background :
Deubiquitinase that specifically cleaves 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains and is involved in NF-kappa-B activation and TNF-alpha-induced necroptosis . Negatively regulates NF-kappa-B activation by deubiquitinating upstream signaling factors . Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation . Negative regulator of Wnt signaling . Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules . Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis . Required for normal cell cycle progress and normal cytokinesis . Inhibits nuclear translocation of NF-kappa-B . Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation . Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells . Negatively regulates TNFRSF11A signaling and osteoclastogenesis . Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation . Ability to remove linear ('Met-1'-linked) polyubiquitin chains regulates innate immunity and TNF-alpha-induced necroptosis: recruited to the LUBAC complex via interaction with SPATA2 and restricts linear polyubiquitin formation on target proteins . Regulates innate immunity by restricting linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation . Involved in TNF-alpha-induced necroptosis by removing linear ('Met-1'-linked) polyubiquitin chains from RIPK1, thereby regulating the kinase activity of RIPK1. Negatively regulates intestinal inflammation by removing 'Lys-63' linked polyubiquitin chain of NLRP6, thereby reducing the interaction between NLRP6 and PYCARD/ASC and formation of the NLRP6 inflammasome. Removes 'Lys-63' linked polyubiquitin chain of MAP3K7, which inhibits phosphorylation and blocks downstream activation of the JNK-p38 kinase cascades . Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of CYLD protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYLD. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
CYLD polyclonal antibody Background :
Deubiquitinase that specifically cleaves 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains and is involved in NF-kappa-B activation and TNF-alpha-induced necroptosis . Negatively regulates NF-kappa-B activation by deubiquitinating upstream signaling factors . Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation . Negative regulator of Wnt signaling . Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules . Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis . Required for normal cell cycle progress and normal cytokinesis . Inhibits nuclear translocation of NF-kappa-B . Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation . Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells . Negatively regulates TNFRSF11A signaling and osteoclastogenesis . Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation . Ability to remove linear ('Met-1'-linked) polyubiquitin chains regulates innate immunity and TNF-alpha-induced necroptosis: recruited to the LUBAC complex via interaction with SPATA2 and restricts linear polyubiquitin formation on target proteins . Regulates innate immunity by restricting linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation . Involved in TNF-alpha-induced necroptosis by removing linear ('Met-1'-linked) polyubiquitin chains from RIPK1, thereby regulating the kinase activity of RIPK1. Negatively regulates intestinal inflammation by removing 'Lys-63' linked polyubiquitin chain of NLRP6, thereby reducing the interaction between NLRP6 and PYCARD/ASC and formation of the NLRP6 inflammasome. Removes 'Lys-63' linked polyubiquitin chain of MAP3K7, which inhibits phosphorylation and blocks downstream activation of the JNK-p38 kinase cascades . Product :
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of CYLD protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYLD. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of CYLD expression in SGC7901 (A) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.