ETS1 monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ETS1 monoclonal antibody Background :
ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence family of transcription factors that share a unique and highly conserved DNA binding domain . ETS-1 plays important roles in vascular development and angiogenesis, and vascular inflammation and remodeling . The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules . In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells . ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity , phosphorylation at its Thr38 site by Ras activates ETS-1 . Product :
Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ETS1 protein. Immunogen :
Recombinant fusion protein of human ETS1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
ETS1 monoclonal antibody Background :
ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence family of transcription factors that share a unique and highly conserved DNA binding domain . ETS-1 plays important roles in vascular development and angiogenesis, and vascular inflammation and remodeling . The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules . In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells . ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity , phosphorylation at its Thr38 site by Ras activates ETS-1 . Product :
Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of ETS1 protein. Immunogen :
Recombinant fusion protein of human ETS1. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of ETS1 expression in MOLT4 (A) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.