SCAP monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
SCAP monoclonal antibody Background :
SREBP cleavage activating protein (SCAP) is a membrane-bound protein essential in regulating sterol regulatory element binding protein (SREBP) processing. In cholesterol-starved cells, SREBPs move from the ER to the Golgi apparatus with the help of SCAP, where the precursor SREBPs are cleaved by site-1 (S1P) and site-2 (S2P) proteases. Released SREBP amino-terminal domains enter the nucleus and bind to sterol response elements located in the promoters of a number of genes responsible for the cholesterol synthesis. Research studies show that the SCAP/SREBP pathway is critical for diabetic fatty liver development and carbohydrate-induced hypertriglyceridemia in mice. In addition, the SCAP/SREBP pathway is required to protect cancer cells from lipotoxicity. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of SCAP protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SCAP. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
SCAP monoclonal antibody Background :
SREBP cleavage activating protein (SCAP) is a membrane-bound protein essential in regulating sterol regulatory element binding protein (SREBP) processing. In cholesterol-starved cells, SREBPs move from the ER to the Golgi apparatus with the help of SCAP, where the precursor SREBPs are cleaved by site-1 (S1P) and site-2 (S2P) proteases. Released SREBP amino-terminal domains enter the nucleus and bind to sterol response elements located in the promoters of a number of genes responsible for the cholesterol synthesis. Research studies show that the SCAP/SREBP pathway is critical for diabetic fatty liver development and carbohydrate-induced hypertriglyceridemia in mice. In addition, the SCAP/SREBP pathway is required to protect cancer cells from lipotoxicity. Product :
Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Recognizes endogenous levels of SCAP protein. Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human SCAP. The exact sequence is proprietary. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of SCAP expression in HT29 (A), LNCaP (B) whole cell lysates.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.