Product Name :
HIF-1α (K377) polyclonal antibody Background :
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, Arnt 1 (also designated HIF-1β), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HIF-1α (K377) polyclonal antibody detects endogenous levels of HIF-1α protein. Immunogen :
Synthetic peptide, corresponding to Human HIF-1α. Conjugate :
Unconjugated Modification :
Unmodification
HIF-1α (K377) polyclonal antibody Background :
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, Arnt 1 (also designated HIF-1β), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HIF-1α (K377) polyclonal antibody detects endogenous levels of HIF-1α protein. Immunogen :
Synthetic peptide, corresponding to Human HIF-1α. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of HIF-1α (K377) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)
-
Western blot (WB) analysis of HIF-1α (K377) polyclonal antibody at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug)
-
Western blot (WB) analysis of HIF-1α (K377) polyclonal antibody at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug)
IL-17-triggered downregulation of miR-497 results in high HIF-1α expression and consequent IL-1β and IL-6 production by astrocytes in EAE mice
PMCID: Pubmed No.:28458392
Localized Delivery of shRNA against PHD2 Protects the Heart from Acute Myocardial Infarction through Ultrasound-Targeted Cationic Microbubble Destruction
PMCID: Pubmed No.:28042316
Norisoboldine, an Anti-Arthritis Alkaloid Isolated from Radix Linderae, Attenuates Osteoclast Differentiation and Inflammatory Bone Erosion in an Aryl Hydrocarbon Receptor-Dependent Manner
PMCID: Pubmed No.:26221077
MiR-223 Suppresses Cell Proliferation by Targeting IGF-1R
PMCID: Pubmed No.:22073238
Cellular FLICE-inhibitory protein protects against cardiac remodelling after myocardial infarction
PMCID: Pubmed No.:22202974
MiR-128 Inhibits Tumor Growth and Angiogenesis by Targeting p70S6K1
PMCID: Pubmed No.:22442669
MiRNA-181b suppresses IGF-1R and functions as a tumor suppressor gene in gliomas
PMCID: Pubmed No.:23431408
miR-21 Contributes to Xenon-conferred Amelioration of Renal Ischemia-Reperfusion Injury in Mice.
PMCID: Pubmed No.:23681145
Intratumor Hypoxia Promotes Immune Tolerance by Inducing Regulatory T Cells via TGF-β1 in Gastric Cancer
PMCID: Pubmed No.:23723999
Insulin Promotes Glucose Consumption via Regulation of miR-99a/mTOR/PKM2 Pathway
PMCID: Pubmed No.:23762265
Expression and significance of HIF-1α in pulmonary fibrosis induced by paraquat
PMCID: Pubmed No.:24048193
Transcatheter arterial embolization promotes liver tumor metastasis by increasing the population of circulating tumor cells
PMCID: Pubmed No.:24235842
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:None
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:24335166
PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer
PMCID: Pubmed No.:24376209
Overexpression of HIF-1α, metallothionein and SLUG is associated with high TNM stage and lymph node metastasis in papillary thyroid carcinoma
PMCID: Pubmed No.:24427353
Antiproliferative activity and therapeutic implications of potassium tris (4-methyl-1-pyrazolyl) borohydride in hepatocellular carcinoma
PMCID: Pubmed No.:24412237
PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer
PMCID: Pubmed No.:24376209
Xuefuzhuyu decoction inhibition of angiogenesis attenuates liver fibrosis induced by CCl4 in mice
PMCID: Pubmed No.:24637190
Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway
PMCID: Pubmed No.:24637576
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells
PMCID: Pubmed No.:24779927
The effect of hyperbaric oxygen on intracephalic angiogenesis in rats with intracerebral hemorrhage
PMCID: Pubmed No.:24836574
MiR-124 governs glioma growth and angiogenesis and enhances chemosensitivity by targeting R-Ras and N-Ras
PMCID: Pubmed No.:24861879
Gli-1 is crucial for hypoxia-induced epithelial-mesenchymal transition and invasion of breast cancer
PMCID: Pubmed No.:25501705
The overexpression and nuclear translocation of Trx-1 during hypoxia confers HepG2 cells resistance to DDP and GL-V9 reverses the resistance by suppressing Trx-1/ref-1 axis.
PMCID: Pubmed No.:25656992
GPER in CAFs regulates hypoxia-driven breast cancer invasion in a CTGF-dependent manner
PMCID: Pubmed No.:25647524
GRP78 is implicated in the modulation of tumor aerobic glycolysis by promoting autophagic degradation of IKKβ
PMCID: Pubmed No.:25748049
Pyruvate kinase M2 accelerates pro-inflammatory cytokine secretion and cell proliferation induced by lipopolysaccharide in colorectal cancer
PMCID: Pubmed No.:25778902
Decreased carboxylesterases expression and hydrolytic activity in type 2 diabetic mice through Akt/mTOR/HIF-1 α/Stra13 pathway
PMCID: Pubmed No.:25801056
Decreased carboxylesterases expression and hydrolytic activity in type 2 diabetic mice through Akt/mTOR/HIF-1α/Stra13 pathway.
PMCID: Pubmed No.:25801056
Norisoboldine, an Anti-Arthritis Alkaloid Isolated from Radix Linderae, Attenuates Osteoclast Differentiation and Inflammatory Bone Erosion in an Aryl Hydrocarbon Receptor-Dependent Manner.
PMCID: Pubmed No.:26221077
Ultrasound-Targeted Microbubble Destruction (UTMD) Assisted Delivery of shRNA against PHD2 into H9C2 Cells
PMCID: Pubmed No.:26267649
MiR-100 Inhibits Osteosarcoma Cell Proliferation, Migration, and Invasion and Enhances Chemosensitivity by Targeting IGFIR
PMCID: Pubmed No.:26306402
Curcumin inhibits cancer-associated fibroblast-driven prostate cancer invasion through MAOA/mTOR/HIF-1α signaling
PMCID: Pubmed No.:26499200
Repression of PES1 expression inhibits growth of gastric cancer
PMCID: Pubmed No.:26423399
Gli-1 is crucial for hypoxia-induced epithelial-mesenchymal transition and invasion of breast cancer
PMCID: Pubmed No.:25501705
TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells
PMCID: Pubmed No.:26462837
Progesterone amplifies oxidative stress signal and promotes NO production via H 2 O 2 in mouse kidney arterial endothelial cells
PMCID: Pubmed No.:26462682
Preventive Effects of the Chinese Herbal Medicine Prescription Tangkuei Decoction for Frigid Extremities on Sciatic Neuropathy in Streptozotocin-Induced Diabetic Rats
PMCID: Pubmed No.:27057201
Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1a signaling axis
PMCID: Pubmed No.:26735336
Gli-1 is crucial for hypoxia-induced epithelial-mesenchymal transition and invasion of breast cancer
PMCID: Pubmed No.:25501705
Transcatheter arterial embolization promotes liver tumor metastasis by increasing the population of circulating tumor cells
PMCID: Pubmed No.:24235842
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:None
Curcumin inhibits cancer-associated fibroblast-driven prostate cancer invasion through MAOA/mTOR/HIF-1α signaling
PMCID: Pubmed No.:26499200
Repression of PES1 expression inhibits growth of gastric cancer
PMCID: Pubmed No.:26423399
Gli-1 is crucial for hypoxia-induced epithelial-mesenchymal transition and invasion of breast cancer
PMCID: Pubmed No.:25501705
TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells
PMCID: Pubmed No.:26462837
Progesterone amplifies oxidative stress signal and promotes NO production via H 2 O 2 in mouse kidney arterial endothelial cells
PMCID: Pubmed No.:26462682
Preventive Effects of the Chinese Herbal Medicine Prescription Tangkuei Decoction for Frigid Extremities on Sciatic Neuropathy in Streptozotocin-Induced Diabetic Rats
PMCID: Pubmed No.:27057201
Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1a signaling axis
PMCID: Pubmed No.:26735336
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells
PMCID: Pubmed No.:24779927
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:24335166
PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer
PMCID: Pubmed No.:24376209
Overexpression of HIF-1α, metallothionein and SLUG is associated with high TNM stage and lymph node metastasis in papillary thyroid carcinoma
PMCID: Pubmed No.:24427353
Antiproliferative activity and therapeutic implications of potassium tris (4-methyl-1-pyrazolyl) borohydride in hepatocellular carcinoma
PMCID: Pubmed No.:24412237
PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer
PMCID: Pubmed No.:24376209
Xuefuzhuyu decoction inhibition of angiogenesis attenuates liver fibrosis induced by CCl4 in mice
PMCID: Pubmed No.:24637190
Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway
PMCID: Pubmed No.:24637576
DNA‐PKcs Deficiency Inhibits Glioblastoma Cell‐Derived Angiogenesis After Ionizing Radiation
PMCID: Pubmed No.:25294801
The role of the miR-31/FIH1 pathway in TGF-β-induced liver fibrosis
PMCID: Pubmed No.:25728779
Metformin inhibits gastric cancer via the inhibition of HIF1α/PKM2 signaling
PMCID: Pubmed No.:26101707
PRL-3 promotes gastric cancer migration and invasion through a NF-κB-HIF-1α-miR-210 axis
PMCID: Pubmed No.:26548949
A positive feedback loop promotes HIF‐1α stability through miR‐210‐mediated suppression of RUNX3 in paraquat‐induced EMT
PMCID: Pubmed No.:28699703
HIF‐1α regulates EMT via the Snail and β‐catenin pathways in paraquat poisoning‐induced early pulmonary fibrosis
PMCID: Pubmed No.:26781174
A novel nano-copper-bearing stainless steel with reduced Cu2+ release only inducing transient foreign body reaction via affecting the activity of NF-κB and Caspase 3
PMCID: Pubmed No.:26604748
GPER in CAFs regulates hypoxia-driven breast cancer invasion in a CTGF-dependent manner
PMCID: Pubmed No.:25647524
Curcumin inhibits cancer-associated fibroblast-driven prostate cancer invasion through MAOA/mTOR/HIF-1α signaling
PMCID: Pubmed No.:26499200
Hypoxia-induced and HIF1α-VEGF-mediated tight junction dysfunction in choriocarcinoma cells: Implications for preeclampsia
PMCID: Pubmed No.:29223764
Progesterone amplifies oxidative stress signal and promotes NO production via H2O2 in mouse kidney arterial endothelial cells
PMCID: Pubmed No.:26462682
GRP78 is implicated in the modulation of tumor aerobic glycolysis by promoting autophagic degradation of IKKβ
PMCID: Pubmed No.:25748049
Ultrasound-Targeted Microbubble Destruction (UTMD) Assisted Delivery of shRNA against PHD2 into H9C2 Cells
PMCID: Pubmed No.:26267649
Curcumin inhibits hypoxia-induced proliferation and invasion of MG-63 osteosarcoma cells via downregulating Notch1
PMCID: Pubmed No.:28138706
Decreased carboxylesterases expression and hydrolytic activity in type 2 diabetic mice through Akt/mTOR/HIF-1α/Stra13 pathway
PMCID: Pubmed No.:25801056
Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase
PMCID: Pubmed No.:29467842
MiRNA-145 increases therapeutic sensibility to gemcitabine treatment of pancreatic adenocarcinoma cells
PMCID: Pubmed No.:27765914
Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling
PMCID: Pubmed No.:27367023
MicroRNA-137 inhibits tumor growth and sensitizes chemosensitivity to paclitaxel and cisplatin in lung cancer
PMCID: Pubmed No.:26989074
Escin Ia suppresses the metastasis of triple-negative breast cancer by inhibiting epithelial-mesenchymal transition via down-regulating LOXL2 expression
PMCID: Pubmed No.:27008697
Gli-1 is crucial for hypoxia-induced epithelial-mesenchymal transition and invasion of breast cancer
PMCID: Pubmed No.:25501705
Repression of PES1 expression inhibits growth of gastric cancer
PMCID: Pubmed No.:26423399
TGF-β1 pathway affects the protein expression of many signaling pathways, markers of liver cancer stem cells, cytokeratins, and TERT in liver cancer HepG2 cells
PMCID: Pubmed No.:26462837
β2-Adrenogenic signaling regulates NNK-induced pancreatic cancer progression via upregulation of HIF-1α
PMCID: Pubmed No.:26497365
Original research: KALRN mutations promote antitumor immunity and immunotherapy response in cancer
PMCID: Pubmed No.:33037113
Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction
PMCID: Pubmed No.:32308762
Anti-tumor effect of local injectable hydrogel-loaded endostatin alone and in combination with radiotherapy for lung cancer
PMCID: Pubmed No.:33427520
Influence of c-Src on hypoxic resistance to paclitaxel in human ovarian cancer cells and reversal of FV-429
PMCID: Pubmed No.:29324735
Intratumoral Injection of Anlotinib Hydrogel Combined With Radiotherapy Reduces Hypoxia in Lewis Lung Carcinoma Xenografts: Assessment by Micro Fluorine-18-fluoromisonidazole Positron Emission Tomography/Computed Tomography Hypoxia Imaging
PMCID: Pubmed No.:33777779
Hypoxia-inducible factor prolyl hydroxylase inhibitor roxadustat (FG-4592) protects against cisplatin-induced acute kidney injury
PMCID: Pubmed No.:29581249
Hydrogen sulfide attenuates renal fibrosis by inducing TET‐dependent DNA demethylation on Klotho promoter
PMCID: Pubmed No.:32729950
Stabilized fermentation product of Cetobacterium somerae improves gut and liver health and antiviral immunity of zebrafish
PMCID: Pubmed No.:34780975
Oroxylin A increases the sensitivity of temozolomide on glioma cells by hypoxia‐inducible factor 1α/hedgehog pathway under hypoxia
PMCID: Pubmed No.:30790292
Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish
PMCID: Pubmed No.:31372513
Atorvastatin attenuates paraquat poisoning-induced epithelial-mesenchymal transition via downregulating hypoxia-inducible factor-1 alpha
PMCID: Pubmed No.:30336147
Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest
PMCID: Pubmed No.:30300703
Enhanced antitumor and anti-angiogenic effects of metronomic Vinorelbine combined with Endostar on Lewis lung carcinoma
PMCID: Pubmed No.:30305062
Activation of TGR5 restores AQP2 expression via the HIF pathway in renal ischemia-reperfusion injury
PMCID: Pubmed No.:33427060
Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway
PMCID: Pubmed No.:29328487
HIF‑1α promotes NLRP3 inflammasome activation in bleomycin‑induced acute lung injury
PMCID: Pubmed No.:31432144
Therapeutic Efficacy of Methazolamide Against Intermittent Hypoxia-Induced Excessive Erythrocytosis in Rats
PMCID: Pubmed No.:29077517
Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps
PMCID: Pubmed No.:35075349
Crosstalk between IL-15Rα+ tumor-associated macrophages and breast cancer cells reduces CD8+ T cell recruitment
PMCID: Pubmed No.:35615815
Mechanism of Shiliu Buxue Syrup for anemia using integrated metabolomics and network pharmacology
PMCID: Pubmed No.:35690102
A Natural Gas Fermentation Bacterial Meal (FeedKind®) as a Functional Alternative Ingredient for Fishmeal in Diet of Largemouth Bass, Micropterus salmoides
PMCID: Pubmed No.:36009198
Neotuberostemonine and tuberostemonine ameliorate pulmonary fibrosis through suppressing TGF-β and SDF-1 secreted by macrophages and fibroblasts via the PI3K-dependent AKT and ERK pathways
PMCID: Pubmed No.:37517820
Ginkgo biloba extract promotes Treg differentiation to ameliorate ischemic stroke via inhibition of HIF-1α/HK2 pathway
PMCID: Pubmed No.:37655539
The effect of stabilized culture of Lactobacillus rhamnosus GCC-3 on gut and liver health, and anti-viral immunity of zebrafish
PMCID: Pubmed No.:37714442
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.