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TGFβ1 (Y369) polyclonal antibody BS1361
  • Western blot (WB) analysis of TGFβ1 (Y369) pAb at 1:5000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
Product NameTGFβ1 (Y369) polyclonal antibody
Catalog No.BS1361
Swiss-ProtP01137
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1:5000~1:10000
BiowMW~ 50 kDa
Alternative NameTransforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP; TGFB1; TGFB; TGF β1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
TGFβ1 (Y369) polyclonal antibody
Background :
Transforming growth factor betas (TGFβs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGFβ1, TGFβ2 and TGFβ3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
TGF β1 (Y369) polyclonal antibody detects endogenous levels of TGF β1 protein.
Immunogen :
Synthetic peptide, corresponding to Human TGFβ1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of TGFβ1 (Y369) pAb at 1:5000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
Angiotensin Type-1 Receptor Blocker Candesartan Inhibits Calcium Oxalate Crystal Deposition in Ethylene Glycol-Treated Rat Kidneys PMCID:    Pubmed No.:21256551 The antifibrotic effects of TGF-[beta] 1 siRNA on hepatic fibrosis in rats PMCID:    Pubmed No.:21600192 Transforming growth factor-ß/Smad signaling function in the aortopathies PMCID:    Pubmed No.:22086576 Disruption of mindin exacerbates cardiac hypertrophy and fibrosis PMCID:    Pubmed No.:22367478 Transforming growth factor beta1/Smad signalling pathway of aortic disorders: histopathological and immunohistochemical studies PMCID:    Pubmed No.:22532183 Upregulation of Nestin in Proximal Tubules may Participate in Cell Migration during Renal Repair PMCID:    Pubmed No.:22993065 Exosomes derived from human umbilical cord mesenchymal stem cells alleviate liver fibrosis PMCID:    Pubmed No.:23002959 Gastric Cancer Exosomes Trigger Differentiation of Umbilical Cord Derived Mesenchymal Stem Cells to Carcinoma-Associated Fibroblasts through TGF-β/Smad Pathway. PMCID:    Pubmed No.:23285052 Elevated transcriptional co-activator p102 mediates angiotensin II type 1 receptor up-regulation and extracellular matrix overproduction in the high glucose-treated rat glomerular mesangial cells and isolated glomeruli PMCID:    Pubmed No.:23376562 Huangkui capsule, an extract from Abelmoschus manihot (L.) medic, ameliorates adriamycin-induced renal inflammation and glomerular injury via inhibiting p38MAPK signaling pathway activity in rats ☆ PMCID:    Pubmed No.:23518420 Induction of Thoracic Aortic Remodeling by Endothelial-Specific Deletion of MicroRNA-21 in Mice PMCID:    Pubmed No.:23527070 Smad3 is the key to transforming growth factor-β1-induced osteoclast differentiation in giant cell tumor of bone PMCID:    Pubmed No.:23690270 Expression and significance of HIF-1α in pulmonary fibrosis induced by paraquat PMCID:    Pubmed No.:24048193 Fasudil hydrochloride hydrate, a Rho-kinase inhibitor, ameliorates hepatic fibrosis in rats with type 2 diabetes PMCID:    Pubmed No.:24438608 Madecassoside Ameliorates Bleomycin‐Induced Pulmonary Fibrosis in Mice by Downregulating Collagen Deposition PMCID:    Pubmed No.:24458872 Microvesicle-mediated delivery of transforming growth factor β1 siRNA for the suppression of tumor growth in mice PMCID:    Pubmed No.:24565517 Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis PMCID:    Pubmed No.:24625224 Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats. PMCID:    Pubmed No.:24831781 Prostacyclin Analogue Beraprost Inhibits Cardiac Fibroblast Proliferation Depending on Prostacyclin Receptor Activation through a TGF β-Smad Signal Pathway PMCID:    Pubmed No.:24852754 Mesenchymal stem cells ameliorate diabetic glomerular fibrosis in vivo and in vitro by inhibiting TGF-β signalling via secretion of bone morphogenetic protein 7. PMCID:    Pubmed No.:24845071 P300-dependent STAT3 acetylation is necessary for angiotensin II-induced pro-fibrotic responses in renal tubular epithelial cells. PMCID:    Pubmed No.:25088002 Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent PMCID:    Pubmed No.:25173938 The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma PMCID:    Pubmed No.:25880619 Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model PMCID:    Pubmed No.:25831208 C1q/tumor necrosis factor-related protein-6 attenuates post-infarct cardiac fibrosis by targeting RhoA/MRTF-A pathway and inhibiting myofibroblast differentiation PMCID:    Pubmed No.:25962701 CTRP3 attenuates post-infarct cardiac fibrosis by targeting Smad3 activation and inhibiting myofibroblast differentiation. PMCID:    Pubmed No.:26138247 An experimental burn wound‐healing study of non‐thermal atmospheric pressure microplasma jet arrays PMCID:    Pubmed No.:26227832 Bone marrow-derived mesenchymal stem cells inhibit the proliferation of hepatic stellate cells by inhibiting the transforming growth factor β pathway PMCID:    Pubmed No.:26458849 Maternal protein restriction that does not have an influence on the birth weight of the offspring induces morphological changes in kidneys reminiscent of phenotypes PMCID:    Pubmed No.:26537761 Insulin deficiency induces rat renal mesangial cell dysfunction via activation of IGF-1/IGF-1R pathway PMCID:    Pubmed No.:26775660 Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir PMCID:    Pubmed No.:25895132 Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages PMCID:    Pubmed No.:27144994 P300-dependent STAT3 acetylation is necessary for angiotensin II-induced pro-fibrotic responses in renal tubular epithelial cells. PMCID:    Pubmed No.:25088002 Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent PMCID:    Pubmed No.:25173938 Bone marrow-derived mesenchymal stem cells inhibit the proliferation of hepatic stellate cells by inhibiting the transforming growth factor β pathway PMCID:    Pubmed No.:26458849 Maternal protein restriction that does not have an influence on the birth weight of the offspring induces morphological changes in kidneys reminiscent of phenotypes PMCID:    Pubmed No.:26537761 Insulin deficiency induces rat renal mesangial cell dysfunction via activation of IGF-1/IGF-1R pathway PMCID:    Pubmed No.:26775660 Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir PMCID:    Pubmed No.:25895132 Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats. PMCID:    Pubmed No.:24831781 Fasudil hydrochloride hydrate, a Rho-kinase inhibitor, ameliorates hepatic fibrosis in rats with type 2 diabetes PMCID:    Pubmed No.:24438608 Madecassoside Ameliorates Bleomycin‐Induced Pulmonary Fibrosis in Mice by Downregulating Collagen Deposition PMCID:    Pubmed No.:24458872 Microvesicle-mediated delivery of transforming growth factor β1 siRNA for the suppression of tumor growth in mice PMCID:    Pubmed No.:24565517 Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis PMCID:    Pubmed No.:24625224 miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression PMCID:    Pubmed No.:28393176 Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages PMCID:    Pubmed No.:27144994 CTRP3 attenuates post-infarct cardiac fibrosis by targeting Smad3 activation and inhibiting myofibroblast differentiation PMCID:    Pubmed No.:26138247 Angiotensin-(1–7) treatment mitigates right ventricular fibrosis as a distinctive feature of diabetic cardiomyopathy PMCID:    Pubmed No.:25724492 FSTL1 as a Potential Mediator of Exercise-Induced Cardioprotection in Post-Myocardial Infarction Rats PMCID:    Pubmed No.:27561749 Spirolactone provides protection from renal fibrosis by inhibiting the endothelial–mesenchymal transition in isoprenaline-induced heart failure in rats PMCID:    Pubmed No.:27217725 Mogroside IIIE, a Novel Anti-Fibrotic Compound, Reduces Pulmonary Fibrosis through Toll-Like Receptor 4 Pathways PMCID:    Pubmed No.:28280123 Protective role of rhapontin in experimental pulmonary fibrosis in vitro and in vivo PMCID:    Pubmed No.:28364627 Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation and Matrix Deposition in Kidney Tissues after Injury PMCID:    Pubmed No.:27313397 Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor β1 signaling pathway in vitro and in vivo PMCID:    Pubmed No.:28412214 Low molecular weight fucoidan ameliorates diabetic nephropathy via inhibiting epithelial-mesenchymal transition and fibrotic processes PMCID:    Pubmed No.:26550455 Paeoniflorin alleviates non-alcoholic steatohepatitis in rats: Involvement with the ROCK/NF-κB pathway PMCID:    Pubmed No.:27351828 The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma PMCID:    Pubmed No.:25880619 Reno-protection of G004, a novel anti-diabetic sulfonylurea in db/db mice PMCID:    Pubmed No.:25943026 Suppressive Effects of Glucose-Dependent Insulinotropic Polypeptide on Cardiac Hypertrophy and Fibrosis in Angiotensin II-Infused Mouse Models PMCID:    Pubmed No.:27375170 Effects of miR-29a and miR-101a Expression on Myocardial Interstitial Collagen Generation After Aerobic Exercise in Myocardial-infarcted Rats PMCID:    Pubmed No.:28577867 Relaxin Ameliorates Renal Fibrosis and Expression of Endothelial Cell Transition Markers in Rats of Isoproterenol-Induced Heart Failure PMCID:    Pubmed No.:28674260 Characterization of spontaneous hydrocephalus development in the young atherosclerosis-prone mice PMCID:    Pubmed No.:28926478 Tangzhiqing Granules Alleviate Podocyte Epithelial-Mesenchymal Transition in Kidney of Diabetic Rats PMCID:    Pubmed No.:28163747 Effect of Huayu Tongluo Herbs on Reduction of Proteinuria via Inhibition of Wnt/β-Catenin Signaling Pathway in Diabetic Rats PMCID:    Pubmed No.:28656052 Urocortin ameliorates diabetic cardiomyopathy in rats via the Akt/GSK-3β signaling pathway PMCID:    Pubmed No.:25667611 Anti-Fibrosis Effect of Relaxin and Spironolactone Combined on Isoprenaline-Induced Myocardial Fibrosis in Rats via Inhibition of Endothelial–Mesenchymal Transition PMCID:    Pubmed No.:28245473 Tangshen Formula Improves Diabetes-Associated Myocardial Fibrosis by Inhibiting TGF-β/Smads and Wnt/β-Catenin Pathways. PMCID:    Pubmed No.:34938743 HSPA12B promotes functional recovery after ischaemic stroke through an eNOS‐dependent mechanism PMCID:    Pubmed No.:29411514 Procyanidin B2 inhibits the activation of hepatic stellate cells and angiogenesis via the Hedgehog pathway during liver fibrosis PMCID:    Pubmed No.:31328391 Application of multiparametric MR imaging to predict the diversification of renal function in miR29a-mediated diabetic nephropathy PMCID:    Pubmed No.:33479331 Potential Effect of Exosomes Derived from Cancer Stem Cells and MSCs on Progression of DEN-Induced HCC in Rats PMCID:    Pubmed No.:30224923 Vitamin D3 supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B PMCID:    Pubmed No.:29953732 C-peptide ameliorates high glucose-induced podocyte dysfunction through the regulation of the Notch and TGF-β signaling pathways PMCID:    Pubmed No.:33901627 The cold-soaking extract of Chinese yam (Dioscorea opposita Thunb.) protects against erectile dysfunction by ameliorating testicular function in hydrocortisone-induced KDS-Yang rats and in oxidatively damaged TM3 cells PMCID:    Pubmed No.:32791294 Nagilactone D ameliorates experimental pulmonary fibrosis in vitro and in vivo via modulating TGF-β/Smad signaling pathway PMCID:    Pubmed No.:31953203 Tetrahydroxy stilbene glycoside regulates TGF-β/fractalkine/CX3CR1 based on network pharmacology in APP/PS1 mouse model PMCID:    Pubmed No.:34509715 Human umbilical cord mesenchymal stem cells inhibit proliferation of hepatic stellate cells in vitro PMCID:    Pubmed No.:29484382 Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment PMCID:    Pubmed No.:29484379 The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson's disease more effectively than the GLP-1 single-receptor agonist NLY01 PMCID:    Pubmed No.:34125470 Maladjustment of β-CGRP/α-CGRP Regulation of AQP5 Promotes Transition of Alveolar Epithelial Cell Apoptosis to Pulmonary Fibrosis PMCID:    Pubmed No.:32746702 Decreased MiR-30a promotes TGF-β1-mediated arachnoid fibrosis in post-hemorrhagic hydrocephalus PMCID:    Pubmed No.:33335750 The Astragaloside IV Derivative LS-102 Ameliorates Obesity-Related Nephropathy PMCID:    Pubmed No.:35308255 Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1 PMCID:    Pubmed No.:35565995 The Histone Demethylase Inhibitor GSK-J4 Is a Therapeutic Target for the Kidney Fibrosis of Diabetic Kidney Disease via DKK1 Modulation PMCID:    Pubmed No.:36012674 Riboflavin ameliorates mitochondrial dysfunction via the AMPK/PGC1α/HO‑1 signaling pathway and attenuates carbon tetrachloride‑induced liver fibrosis in rats PMCID:    Pubmed No.:36160891 Nasal irrigation with licorice extract (Glycyrrhiza glabra) in treating nasal polyps by reducing fibroblast differentiation and extracellular matrix production in TGF-β1-stimulated nasal polyp-derived fibroblasts by inhibiting the MAPK/ERK-1/2 pathway – an in vitro and in clinic study PMCID:    Pubmed No.:36447209 Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease PMCID:    Pubmed No.:37108841 Exosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis PMCID:    Pubmed No.:37525152 Effect of sequential release of sirolimus and rosuvastatin using silk fibroin microneedle to prevent intimal hyperplasia PMCID:    Pubmed No.:37837879
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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