Product Name :
TGFβ1 (Y369) polyclonal antibody Background :
Transforming growth factor betas (TGFβs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGFβ1, TGFβ2 and TGFβ3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TGF β1 (Y369) polyclonal antibody detects endogenous levels of TGF β1 protein. Immunogen :
Synthetic peptide, corresponding to Human TGFβ1. Conjugate :
Unconjugated Modification :
Unmodification
TGFβ1 (Y369) polyclonal antibody Background :
Transforming growth factor betas (TGFβs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGFβ1, TGFβ2 and TGFβ3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TGF β1 (Y369) polyclonal antibody detects endogenous levels of TGF β1 protein. Immunogen :
Synthetic peptide, corresponding to Human TGFβ1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of TGFβ1 (Y369) pAb at 1:5000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
Angiotensin Type-1 Receptor Blocker Candesartan Inhibits Calcium Oxalate Crystal Deposition in Ethylene Glycol-Treated Rat Kidneys
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The antifibrotic effects of TGF-[beta] 1 siRNA on hepatic fibrosis in rats
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Transforming growth factor-ß/Smad signaling function in the aortopathies
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Disruption of mindin exacerbates cardiac hypertrophy and fibrosis
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Transforming growth factor beta1/Smad signalling pathway of aortic disorders: histopathological and immunohistochemical studies
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Upregulation of Nestin in Proximal Tubules may Participate in Cell Migration during Renal Repair
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Exosomes derived from human umbilical cord mesenchymal stem cells alleviate liver fibrosis
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Gastric Cancer Exosomes Trigger Differentiation of Umbilical Cord Derived Mesenchymal Stem Cells to Carcinoma-Associated Fibroblasts through TGF-β/Smad Pathway.
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Elevated transcriptional co-activator p102 mediates angiotensin II type 1 receptor up-regulation and extracellular matrix overproduction in the high glucose-treated rat glomerular mesangial cells and isolated glomeruli
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Huangkui capsule, an extract from Abelmoschus manihot (L.) medic, ameliorates adriamycin-induced renal inflammation and glomerular injury via inhibiting p38MAPK signaling pathway activity in rats ☆
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Induction of Thoracic Aortic Remodeling by Endothelial-Specific Deletion of MicroRNA-21 in Mice
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Smad3 is the key to transforming growth factor-β1-induced osteoclast differentiation in giant cell tumor of bone
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Expression and significance of HIF-1α in pulmonary fibrosis induced by paraquat
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Fasudil hydrochloride hydrate, a Rho-kinase inhibitor, ameliorates hepatic fibrosis in rats with type 2 diabetes
PMCID: Pubmed No.:24438608
Madecassoside Ameliorates Bleomycin‐Induced Pulmonary Fibrosis in Mice by Downregulating Collagen Deposition
PMCID: Pubmed No.:24458872
Microvesicle-mediated delivery of transforming growth factor β1 siRNA for the suppression of tumor growth in mice
PMCID: Pubmed No.:24565517
Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis
PMCID: Pubmed No.:24625224
Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats.
PMCID: Pubmed No.:24831781
Prostacyclin Analogue Beraprost Inhibits Cardiac Fibroblast Proliferation Depending on Prostacyclin Receptor Activation through a TGF β-Smad Signal Pathway
PMCID: Pubmed No.:24852754
Mesenchymal stem cells ameliorate diabetic glomerular fibrosis in vivo and in vitro by inhibiting TGF-β signalling via secretion of bone morphogenetic protein 7.
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P300-dependent STAT3 acetylation is necessary for angiotensin II-induced pro-fibrotic responses in renal tubular epithelial cells.
PMCID: Pubmed No.:25088002
Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent
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The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma
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Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model
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C1q/tumor necrosis factor-related protein-6 attenuates post-infarct cardiac fibrosis by targeting RhoA/MRTF-A pathway and inhibiting myofibroblast differentiation
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CTRP3 attenuates post-infarct cardiac fibrosis by targeting Smad3 activation and inhibiting myofibroblast differentiation.
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An experimental burn wound‐healing study of non‐thermal atmospheric pressure microplasma jet arrays
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Bone marrow-derived mesenchymal stem cells inhibit the proliferation of hepatic stellate cells by inhibiting the transforming growth factor β pathway
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Maternal protein restriction that does not have an influence on the birth weight of the offspring induces morphological changes in kidneys reminiscent of phenotypes
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Insulin deficiency induces rat renal mesangial cell dysfunction via activation of IGF-1/IGF-1R pathway
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Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir
PMCID: Pubmed No.:25895132
Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages
PMCID: Pubmed No.:27144994
P300-dependent STAT3 acetylation is necessary for angiotensin II-induced pro-fibrotic responses in renal tubular epithelial cells.
PMCID: Pubmed No.:25088002
Ageing related periostin expression increase from cardiac fibroblasts promotes cardiomyocytes senescent
PMCID: Pubmed No.:25173938
Bone marrow-derived mesenchymal stem cells inhibit the proliferation of hepatic stellate cells by inhibiting the transforming growth factor β pathway
PMCID: Pubmed No.:26458849
Maternal protein restriction that does not have an influence on the birth weight of the offspring induces morphological changes in kidneys reminiscent of phenotypes
PMCID: Pubmed No.:26537761
Insulin deficiency induces rat renal mesangial cell dysfunction via activation of IGF-1/IGF-1R pathway
PMCID: Pubmed No.:26775660
Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir
PMCID: Pubmed No.:25895132
Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats.
PMCID: Pubmed No.:24831781
Fasudil hydrochloride hydrate, a Rho-kinase inhibitor, ameliorates hepatic fibrosis in rats with type 2 diabetes
PMCID: Pubmed No.:24438608
Madecassoside Ameliorates Bleomycin‐Induced Pulmonary Fibrosis in Mice by Downregulating Collagen Deposition
PMCID: Pubmed No.:24458872
Microvesicle-mediated delivery of transforming growth factor β1 siRNA for the suppression of tumor growth in mice
PMCID: Pubmed No.:24565517
Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis
PMCID: Pubmed No.:24625224
miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression
PMCID: Pubmed No.:28393176
Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages
PMCID: Pubmed No.:27144994
CTRP3 attenuates post-infarct cardiac fibrosis by targeting Smad3 activation and inhibiting myofibroblast differentiation
PMCID: Pubmed No.:26138247
Angiotensin-(1–7) treatment mitigates right ventricular fibrosis as a distinctive feature of diabetic cardiomyopathy
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FSTL1 as a Potential Mediator of Exercise-Induced Cardioprotection in Post-Myocardial Infarction Rats
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Spirolactone provides protection from renal fibrosis by inhibiting the endothelial–mesenchymal transition in isoprenaline-induced heart failure in rats
PMCID: Pubmed No.:27217725
Mogroside IIIE, a Novel Anti-Fibrotic Compound, Reduces Pulmonary Fibrosis through Toll-Like Receptor 4 Pathways
PMCID: Pubmed No.:28280123
Protective role of rhapontin in experimental pulmonary fibrosis in vitro and in vivo
PMCID: Pubmed No.:28364627
Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation and Matrix Deposition in Kidney Tissues after Injury
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Chinese medicine Bu-Fei decoction attenuates epithelial-mesenchymal transition of non-small cell lung cancer via inhibition of transforming growth factor β1 signaling pathway in vitro and in vivo
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Low molecular weight fucoidan ameliorates diabetic nephropathy via inhibiting epithelial-mesenchymal transition and fibrotic processes
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Paeoniflorin alleviates non-alcoholic steatohepatitis in rats: Involvement with the ROCK/NF-κB pathway
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The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma
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Reno-protection of G004, a novel anti-diabetic sulfonylurea in db/db mice
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Suppressive Effects of Glucose-Dependent Insulinotropic Polypeptide on Cardiac Hypertrophy and Fibrosis in Angiotensin II-Infused Mouse Models
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Effects of miR-29a and miR-101a Expression on Myocardial Interstitial Collagen Generation After Aerobic Exercise in Myocardial-infarcted Rats
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Relaxin Ameliorates Renal Fibrosis and Expression of Endothelial Cell Transition Markers in Rats of Isoproterenol-Induced Heart Failure
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Characterization of spontaneous hydrocephalus development in the young atherosclerosis-prone mice
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Tangzhiqing Granules Alleviate Podocyte Epithelial-Mesenchymal Transition in Kidney of Diabetic Rats
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Effect of Huayu Tongluo Herbs on Reduction of Proteinuria via Inhibition of Wnt/β-Catenin Signaling Pathway in Diabetic Rats
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Urocortin ameliorates diabetic cardiomyopathy in rats via the Akt/GSK-3β signaling pathway
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Anti-Fibrosis Effect of Relaxin and Spironolactone Combined on Isoprenaline-Induced Myocardial Fibrosis in Rats via Inhibition of Endothelial–Mesenchymal Transition
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Tangshen Formula Improves Diabetes-Associated Myocardial Fibrosis by Inhibiting TGF-β/Smads and Wnt/β-Catenin Pathways.
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HSPA12B promotes functional recovery after ischaemic stroke through an eNOS‐dependent mechanism
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Procyanidin B2 inhibits the activation of hepatic stellate cells and angiogenesis via the Hedgehog pathway during liver fibrosis
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Application of multiparametric MR imaging to predict the diversification of renal function in miR29a-mediated diabetic nephropathy
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Potential Effect of Exosomes Derived from Cancer Stem Cells and MSCs on Progression of DEN-Induced HCC in Rats
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Vitamin D3 supplementation improves testicular function in diabetic rats through peroxisome proliferator‐activated receptor‐γ/transforming growth factor‐beta 1/nuclear factor‐kappa B
PMCID: Pubmed No.:29953732
C-peptide ameliorates high glucose-induced podocyte dysfunction through the regulation of the Notch and TGF-β signaling pathways
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The cold-soaking extract of Chinese yam (Dioscorea opposita Thunb.) protects against erectile dysfunction by ameliorating testicular function in hydrocortisone-induced KDS-Yang rats and in oxidatively damaged TM3 cells
PMCID: Pubmed No.:32791294
Nagilactone D ameliorates experimental pulmonary fibrosis in vitro and in vivo via modulating TGF-β/Smad signaling pathway
PMCID: Pubmed No.:31953203
Tetrahydroxy stilbene glycoside regulates TGF-β/fractalkine/CX3CR1 based on network pharmacology in APP/PS1 mouse model
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Human umbilical cord mesenchymal stem cells inhibit proliferation of hepatic stellate cells in vitro
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Early intervention with mesenchymal stem cells prevents nephropathy in diabetic rats by ameliorating the inflammatory microenvironment
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The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson's disease more effectively than the GLP-1 single-receptor agonist NLY01
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Maladjustment of β-CGRP/α-CGRP Regulation of AQP5 Promotes Transition of Alveolar Epithelial Cell Apoptosis to Pulmonary Fibrosis
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Decreased MiR-30a promotes TGF-β1-mediated arachnoid fibrosis in post-hemorrhagic hydrocephalus
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The Astragaloside IV Derivative LS-102 Ameliorates Obesity-Related Nephropathy
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Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1
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The Histone Demethylase Inhibitor GSK-J4 Is a Therapeutic Target for the Kidney Fibrosis of Diabetic Kidney Disease via DKK1 Modulation
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Riboflavin ameliorates mitochondrial dysfunction via the AMPK/PGC1α/HO‑1 signaling pathway and attenuates carbon tetrachloride‑induced liver fibrosis in rats
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Nasal irrigation with licorice extract (Glycyrrhiza glabra) in treating nasal polyps by reducing fibroblast differentiation and extracellular matrix production in TGF-β1-stimulated nasal polyp-derived fibroblasts by inhibiting the MAPK/ERK-1/2 pathway – an in vitro and in clinic study
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Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease
PMCID: Pubmed No.:37108841
Exosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis
PMCID: Pubmed No.:37525152
Effect of sequential release of sirolimus and rosuvastatin using silk fibroin microneedle to prevent intimal hyperplasia
PMCID: Pubmed No.:37837879
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.