Product Name :
p38 (V318) polyclonal antibody Background :
MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p38 (V318) polyclonal antibody detects endogenous levels of p38 protein. Immunogen :
Synthetic peptide, corresponding to Human p38. Conjugate :
Unconjugated Modification :
Unmodification
p38 (V318) polyclonal antibody Background :
MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p38 (V318) polyclonal antibody detects endogenous levels of p38 protein. Immunogen :
Synthetic peptide, corresponding to Human p38. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of p38 (V318) polyclonal antibody at 1:1000 dillution Lane1:HEK293T whole cell lysate(36μg) Lane2:NIH-3T3 whole cell lysate(38.8μg) Lane3:HepG2 whole cell lysate(36.8μg)
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Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
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Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
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Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #AP0424.
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Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
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IL‐17 regulates the expressions of RANKL and OPG in human periodontal ligament cells via TRAF6/TBK1‐JNK/NF‐κB pathways
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Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB)
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Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats.
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The protective effects of urocortin1 against intracerebral hemorrhage by activating JNK1/2 and p38 phosphorylation and further increasing VEGF via corticotropin-releasing factor receptor 2.
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Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway
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Protective effects of dioscin against alcohol-induced liver injury
PMCID: Pubmed No.:24146112
An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer
PMCID: Pubmed No.:24274457
Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms
PMCID: Pubmed No.:26604089
Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways
PMCID: Pubmed No.:26503828
Norisoboldine ameliorates DSS-induced ulcerative colitis in mice through induction of regulatory T cells in colons.
PMCID: Pubmed No.:26363976
DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
PMCID: Pubmed No.:26456500
Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion.
PMCID: Pubmed No.:26849940
Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis
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Role of p38, ERK1/2, focal adhesion kinase, RhoA/ROCK and cytoskeleton in the adipogenesis of human mesenchymal stem cells.
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Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway
PMCID: Pubmed No.:24406296
LW-214, a newly synthesized flavonoid, induces intrinsic apoptosis pathway by down-regulating Trx-1 in MCF-7 human breast cells
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Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation
PMCID: Pubmed No.:24491258
The DPP-4 inhibitor sitagliptin attenuates the progress of atherosclerosis in apolipoprotein-E-knockout mice via AMPK-and MAPK-dependent mechanisms
PMCID: Pubmed No.:24490809
Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways
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ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.