Product Name :
JNK1/2/3 (phospho-T183/Y185) polyclonal antibody Background :
The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-JNK1/2/3 (T183/Y185) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183 and Tyr185. Immunogen :
Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183 and Tyrosine 185. Conjugate :
Unconjugated Modification :
Phosphorylation
JNK1/2/3 (phospho-T183/Y185) polyclonal antibody Background :
The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-JNK1/2/3 (T183/Y185) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183 and Tyr185. Immunogen :
Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183 and Tyrosine 185. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-JNK1/2/3 (T183/Y185) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:sp2/0 cell lysate treated with UV Lane3:PC12 cell lysate treated with UV
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Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
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Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
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Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.