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Rsk-1 (T353) polyclonal antibody BS1448
  • Western blot (WB) analysis of Rsk-1 (T353) pAb at 1:2000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug)
  • Immunoprecipitation of HCT116 cell lysates using Rsk-1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 1 is 30% input. The western blot was probed using Rsk-1 pAb #BS1448.
  • Immunoprecipitation of HCT116 cell lysates using Rsk-1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 1 is 30% input. The western blot was probed using Rsk-1 pAb #BS1448.
Product NameRsk-1 (T353) polyclonal antibody
Catalog No.BS1448
Swiss-ProtQ15418
Host Rabbit
ReactivityHuman,Rat
ApplicationsWB IP IF
Application_allWB: 1:2000~1:5000 IF: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 85 kDa
Alternative NameRibosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; RPS6KA1; MAPKAPK1A; RSK1; S6K-α1; S6K α1; S6Kα1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
Rsk-1 (T353) polyclonal antibody
Background :
The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Rsk-1 (T353) polyclonal antibody detects endogenous levels of Rsk-1 protein.
Immunogen :
Synthetic peptide, corresponding to Human Rsk-1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Rsk-1 (T353) pAb at 1:2000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug)
  • Immunoprecipitation of HCT116 cell lysates using Rsk-1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 1 is 30% input. The western blot was probed using Rsk-1 pAb #BS1448.
  • Immunoprecipitation of HCT116 cell lysates using Rsk-1 pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 1 is 30% input. The western blot was probed using Rsk-1 pAb #BS1448.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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