Product Name :
Bax (C62) polyclonal antibody Background :
The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Bax (C62) polyclonal antibody detects endogenous levels of Bax protein. Immunogen :
Synthetic peptide, corresponding to Human Bax. Conjugate :
Unconjugated Modification :
Unmodification
Bax (C62) polyclonal antibody Background :
The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Bax (C62) polyclonal antibody detects endogenous levels of Bax protein. Immunogen :
Synthetic peptide, corresponding to Human Bax. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Bax (S4) polyclonal antibody at 1:500 dilution LaneA:The Brain cord tissue lysate of Mouse(40ug) LaneB:The Brain tissue lysate of Rat(40ug) LaneC:A2780 whole cell lysate(40ug) LaneD:MCF-7 whole cell lysate(40ug)
-
Immunofluorescence analysis of A549 cells using Bax (C62) pAb at dilution of 1:50.
-
Immunofluorescence analysis of A549 cells using Bax (C62) pAb at dilution of 1:50.
The Long Intergenic Noncoding RNA UFC1, a Target of MicroRNA 34a, Interacts With the mRNA Stabilizing Protein HuR to Increase Levels of β-Catenin in HCC Cells
PMCID: Pubmed No.:25449213
Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells
PMCID: Pubmed No.:27463126
Involvement of JNK and P53 Activation in G2/M Cell Cycle Arrest and Apoptosis induced by Titanium Dioxide Nanoparticles in neuron cells
PMCID: Pubmed No.:20863874
Small interfering RNA targeting integrin-linked kinase inhibited the growth and induced apoptosis in human bladder cancer cells
PMCID: Pubmed No.:21601006
2,5-Hexanedione induces human ovarian granulosa cell apoptosis through BCL-2, BAX, and CASPASE-3 signaling pathways
PMCID: Pubmed No.:21901545
Inhibition of activated Stat3 reverses drug resistance to chemotherapeutic agents in gastric cancer cells
PMCID: Pubmed No.:22104727
(S)-ZJM-289, a nitric oxide-releasing derivative of 3-n-butylphthalide, protects against ischemic neuronal injury by attenuating mitochondrial dysfunction and associated cell death
PMCID: Pubmed No.:22142531
Negative regulation-resistant p53 variant enhances oncolytic adenoviral gene therapy
PMCID: Pubmed No.:22248367
A natural coumarin derivative esculetin offers neuroprotection on cerebral ischemia/reperfusion injury in mice
PMCID: Pubmed No.:22458555
Microcystin-LR Induces Ceramide to Regulate PP2A and Destabilize Cytoskeleton in HEK293 Cells
PMCID: Pubmed No.:22523228
Down regulation of differentiated embryonic chondrocytes 1 (DEC1) is involved in 8-methoxypsoralen-induced apoptosis in HepG2 cells
PMCID: Pubmed No.:22796345
Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through fas- and mitochondria-mediated pathways†
PMCID: Pubmed No.:22821714
Akebia Saponin D attenuates amyloid β-induced cognitive deficits and inflammatory response in rats: Involvement of Akt/NF-κB pathway
PMCID: Pubmed No.:22963993
Neurotoxic potential of iron oxide nanoparticles in the rat brain striatum and hippocampus
PMCID: Pubmed No.:22995439
Phycocyanin may suppress d-galactose-induced human lens epithelial cell apoptosis through mitochondrial and unfolded protein response pathways
PMCID: Pubmed No.:23036742
Paclitaxel-Fe3O4 nanoparticles inhibit growth of CD138- CD34- tumor stem-like cells in multiple myeloma-bearing mice
PMCID: Pubmed No.:23610522
Effect of bone marrow mesenchymal stem cells from blastic phase chronic myelogenous leukemia on the growth and apoptosis of leukemia cells
PMCID: Pubmed No.:23733230
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:None
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:24335166
Melatonin treatment induces interplay of apoptosis, autophagy, and senescence in human colorectal cancer cells
PMCID: Pubmed No.:24484372
Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
PMCID: Pubmed No.:24613208
Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway.
PMCID: Pubmed No.:24769129
Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways
PMCID: Pubmed No.:25270871
Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats
PMCID: Pubmed No.:26074995
LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells
PMCID: Pubmed No.:25945460
8-Methoxypsoralen Induces Intrinsic Apoptosis in HepG2 Cells: Involvement of Reactive Oxygen Species Generation and ERK1/2 Pathway Inhibition
PMCID: Pubmed No.:26316083
Racemic oleracein E increases the survival rate and attenuates memory impairment in D-galactose/NaNO 2-induced senescent mice
PMCID: Pubmed No.:27064004
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways
PMCID: Pubmed No.:25270871
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:None
Racemic oleracein E increases the survival rate and attenuates memory impairment in D-galactose/NaNO 2-induced senescent mice
PMCID: Pubmed No.:27064004
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway.
PMCID: Pubmed No.:24769129
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:24335166
Melatonin treatment induces interplay of apoptosis, autophagy, and senescence in human colorectal cancer cells
PMCID: Pubmed No.:24484372
Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
PMCID: Pubmed No.:24613208
Racemic oleracein E increases the survival rate and attenuates memory impairment in D-galactose/NaNO2-induced senescent mice
PMCID: Pubmed No.:27064004
Phenolic alkaloid oleracein E attenuates oxidative stress and neurotoxicity in AlCl3-treated mice
PMCID: Pubmed No.:29054451
Concentration-dependent differential effects of an epothilone analog on cell cycle and p53 signaling
PMCID: Pubmed No.:26177745
LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells
PMCID: Pubmed No.:25945460
Celastrol suppresses the proliferation of lung adenocarcinoma cells by regulating microRNA-24 and microRNA-181b
PMCID: Pubmed No.:29434967
Downregulation of Foxc2 enhances apoptosis induced by 5-fluorouracil through activation of MAPK and AKT pathways in colorectal cancer
PMCID: Pubmed No.:26893778
Saponin 6 derived from Anemone taipaiensis induces U87 human malignant glioblastoma cell apoptosis via regulation of Fas and Bcl‑2 family proteins
PMCID: Pubmed No.:27175997
Imiquimod inhibits the growth of SGC‑7901 cells in vitro through induction of autophagy and apoptosis
PMCID: Pubmed No.:26549648
Rhus javanica Linn protects against hydrogen peroxide‑induced toxicity in human Chang liver cells via attenuation of oxidative stress and apoptosis signaling
PMCID: Pubmed No.:26648020
Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats
PMCID: Pubmed No.:26074995
Association between integrin‑linked kinase and hyperthermia in oral squamous cell carcinoma
PMCID: Pubmed No.:29250172
Effect of vascular endothelial growth factor siRNA and wild‑type p53 co‑expressing plasmid in MDA‑MB‑231 cells
PMCID: Pubmed No.:26573068
Type II cGMP‑dependent protein kinase inhibits the migration, invasion and proliferation of several types of human cancer cells
PMCID: Pubmed No.:28849123
C1 inhibitor‑mediated myocardial protection from chronic intermittent hypoxia‑induced injury
PMCID: Pubmed No.:27698713
RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells
PMCID: Pubmed No.:26063610
Resveratrol improves human umbilical cord-derived mesenchymal stem cells repair for cisplatin-induced acute kidney injury
PMCID: Pubmed No.:30237401
Potent effects of dioscin against hepatocellular carcinoma through regulating TP53‐induced glycolysis and apoptosis regulator (TIGAR)‐mediated apoptosis, autophagy, and DNA damage
PMCID: Pubmed No.:30710454
A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway
PMCID: Pubmed No.:34887997
The Roles of FGF21 and ALCAT1 in Aerobic Exercise-Induced Cardioprotection of Postmyocardial Infarction Mice
PMCID: Pubmed No.:34777697
Differential toxicities of triptolide to immortalized podocytes and the podocytes in vivo
PMCID: Pubmed No.:30551497
Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice
PMCID: Pubmed No.:34084135
Pyridostigmine alleviates cardiac dysfunction via improving mitochondrial cristae shape in a mouse model of metabolic syndrome
PMCID: Pubmed No.:30633969
Fruiting body polysaccharides of Hericium erinaceus induce apoptosis in human colorectal cancer cells via ROS generation mediating caspase-9-dependent signaling pathways
PMCID: Pubmed No.:32573644
A novel drug-free strategy of nano-pulse stimulation sequence (NPSS) in oral cancer therapy: In vitro and in vivo study
PMCID: Pubmed No.:29719274
Reversal Effects of Bound Polyphenol from Foxtail Millet Bran on Multidrug Resistance in Human HCT-8/Fu Colorectal Cancer Cell
PMCID: Pubmed No.:29730933
Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells
PMCID: Pubmed No.:33663206
Targeting PI3K/Akt/Nrf2 pathway by glabridin alleviates acetaminophen-induced hepatic injury in rats
PMCID: Pubmed No.:33932887
The RhoA/ROCK pathway mediates high glucose-induced cardiomyocyte apoptosis via oxidative stress, JNK, and p38MAPK pathways
PMCID: Pubmed No.:29745021
Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway
PMCID: Pubmed No.:31523192
The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling
PMCID: Pubmed No.:31884423
HucMSC exosome-delivered 14-3-3ζ alleviates ultraviolet radiation-induced photodamage via SIRT1 pathway modulation
PMCID: Pubmed No.:33882455
Toosendanin Shows Potent Efficacy Against Human Ovarian Cancer through Caspase-Dependent Mitochondrial Apoptotic Pathway
PMCID: Pubmed No.:34521317
Mineralocorticoid receptor deficiency improves the therapeutic effects of mesenchymal stem cells for myocardial infarction via enhanced cell survival
PMCID: Pubmed No.:30549184
A pentacyclic triterpene derivative possessing polyhydroxyl ring A suppresses growth of HeLa cells by reactive oxygen species-dependent NF-κB pathway
PMCID: Pubmed No.:30153443
The neuroprotective effect of phillyrin in intracerebral hemorrhagic mice is produced by activation of the Nrf2 signaling pathway
PMCID: Pubmed No.:34425100
Long noncoding RNA DINO (damage induced noncoding) represses the development of gastric cancer by modulating p21 and Bcl-2 Associated X Protein (Bax) expression
PMCID: Pubmed No.:30775800
Long noncoding RNA LINC00961 inhibits cell proliferation and induces cell apoptosis in human non–small cell lung cancer
PMCID: Pubmed No.:30010215
Diterpene Ginkgolides Meglumine Injection inhibits apoptosis induced by optic nerve crush injury via modulating MAPKs signaling pathways in retinal ganglion cells
PMCID: Pubmed No.:34181957
Hederagenin Attenuates Cerebral Ischaemia/Reperfusion Injury by Regulating MLK3 Signalling
PMCID: Pubmed No.:32848779
Protective effects of Salidroside on cardiac function in mice with myocardial infarction
PMCID: Pubmed No.:31792327
Profiles of messenger RNAs and MicroRNAs in hypoxia-induced hepatic stellate cells
PMCID: Pubmed No.:34734003
LncRNA-GAS5 promotes spinal cord repair and the inhibition of neuronal apoptosis via the transplantation of 3D printed scaffold loaded with induced pluripotent stem cell-derived neural stem cells
PMCID: Pubmed No.:34350246
Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc
PMCID: Pubmed No.:30186330
The novel GLP-1/GIP dual agonist DA3-CH is more effective than liraglutide in reducing endoplasmic reticulum stress in diabetic rats with cerebral ischemia-reperfusion injury
PMCID: Pubmed No.:33500109
Ursolic acid induces apoptosis and anoikis in colorectal carcinoma RKO cells
PMCID: Pubmed No.:33549076
LncRNA SNHG6 Inhibits Apoptosis by Regulating EZH2 Expression via the Sponging of MiR-101-3p in Esophageal Squamous-Cell Carcinoma
PMCID: Pubmed No.:33192074
Echinocystic acid provides a neuroprotective effect via the PI3K/AKT pathway in intracerebral haemorrhage mice
PMCID: Pubmed No.:32055597
Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model
PMCID: Pubmed No.:33433498
Rosmarinic acid elicits neuroprotection in ischemic stroke via Nrf2 and heme oxygenase 1 signaling
PMCID: Pubmed No.:30323140
Devazepide suppresses cell proliferation and migration, and induces apoptosis in bladder carcinoma
PMCID: Pubmed No.:34159092
Rosiglitazone attenuates cell apoptosis through antioxidative and anti-apoptotic pathways in the hippocampi of spontaneously hypertensive rats
PMCID: Pubmed No.:30483729
Induction of microRNA‑let‑7a inhibits lung adenocarcinoma cell growth by regulating cyclin D1
PMCID: Pubmed No.:30066899
Adipokine zinc-α2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the β3-AR/PKA/CREB pathway
PMCID: Pubmed No.:31260855
Mitochondrial targeting of TR3 is involved in TPA induced apoptosis in breast cancer cells
PMCID: Pubmed No.:30641217
Anticancer roles of let‑7f‑1‑3p in non‑small cell lung cancer via direct targeting of integrin β1
PMCID: Pubmed No.:34630660
Effect of Heat Treatment on the Anticancer Activity of Houttuynia cordata Thunb Aerial Stem Extract in Human Gastric Cancer SGC-7901 Cells
PMCID: Pubmed No.:32180441
-Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells
PMCID: Pubmed No.:30363706
The novel GLP-1/GIP dual receptor agonist DA3-CH is neuroprotective in the pilocarpine-induced epileptogenesis rat model
PMCID: Pubmed No.:31121474
Post-treatment with the GLP-1 analogue liraglutide alleviate chronic inflammation and mitochondrial stress induced by Status epilepticus
PMCID: Pubmed No.:29549796
IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway
PMCID: Pubmed No.:32945463
Semaglutide-mediated protection against Aβ correlated with enhancement of autophagy and inhibition of apotosis
PMCID: Pubmed No.:33222922
Effect of Oleanolic Acid on Apoptosis and Autophagy of SMMC-7721 Hepatoma Cells
PMCID: Pubmed No.:32424110
Ethanol Extract of Hizikia fusiforme Induces Apoptosis in B16F10 Mouse Melanoma Cells through ROS-Dependent Inhibition of the PI3K/Akt Signaling Pathway
PMCID: Pubmed No.:32458633
Quercetin protects human liver cells from o,p’-DDT-induced toxicity by suppressing Nrf2 and NADPH oxidase-regulated ROS production
PMCID: Pubmed No.:35122929
Delivery of miR-3529-3p using MnO2-SiO2-APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A
PMCID: Pubmed No.:36808485
Radiosensitization of Nasopharyngeal Carcinoma by Graphene Oxide Nanosheets to Reduce Bcl-2 Level
PMCID: Pubmed No.:36913208
QHRD106 ameliorates ischemic stroke injury as a long-acting tissue kallikrein preparation
PMCID: Pubmed No.:37496671
Pioglitazone protects PC12 cells against oxidative stress injury: An in vitro study of its antiapoptotic effects via the PPARγ pathway
PMCID: Pubmed No.:37854503
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.