mTOR (S2442) polyclonal antibody 
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Product Name :
mTOR (S2442) polyclonal antibody Background :
mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
mTOR (S2442) polyclonal antibody detects endogenous levels of mTOR protein. Immunogen :
Synthetic peptide, corresponding to Human mTOR. Conjugate :
Unconjugated Modification :
Unmodification
mTOR (S2442) polyclonal antibody Background :
mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
mTOR (S2442) polyclonal antibody detects endogenous levels of mTOR protein. Immunogen :
Synthetic peptide, corresponding to Human mTOR. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of mTOR (S2442) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug) -
Immunoprecipitation of HEK293T cell lysates using mTOR (S2442) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using mTOR (S2442) pAb #BS3611. -
Immunoprecipitation of HEK293T cell lysates using mTOR (S2442) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using mTOR (S2442) pAb #BS3611.
Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway.
PMCID: Pubmed No.:22139427
E Platinum, a newly synthesized platinum compound, induces autophagy via inhibiting phosphorylation of mTOR in gastric carcinoma BGC-823 cells
PMCID: Pubmed No.:22322152
Disruption of mindin exacerbates cardiac hypertrophy and fibrosis
PMCID: Pubmed No.:22367478
Beclin 1-mediated autophagy in hepatocellular carcinoma cells: Implication in anticancer efficiency of oroxylin A via inhibition of mTOR signaling
PMCID: Pubmed No.:22560876
MiR-145 inhibits tumor angiogenesis and growth by N-RAS and VEGF
PMCID: Pubmed No.:22592534
Cucurmosin kills human pancreatic cancer SW-1990 cells in vitro and in vivo.
PMCID: Pubmed No.:23157594
Cucurmosin induces the apoptosis of human pancreatic cancer CFPAC-1 cells by inactivating the PDGFR-b signalling pathway
PMCID: Pubmed No.:23950591
Peroxisome proliferator-activated receptor-γ interrupts angiogenic signal transduction via transrepression of platelet-derived growth factor-β receptor in hepatic stellate cells
PMCID: Pubmed No.:24259663
Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway
PMCID: Pubmed No.:24366110
Luteolin is effective in the non‐small‐cell lung cancer model with L858R/T790M EGFR mutation and erlotinib resistance
PMCID: Pubmed No.:24471765
Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions
PMCID: Pubmed No.:24504086
Rapamycin attenuates visible light-induced injury in retinal photoreceptor cells via inhibiting endoplasmic reticulum stress
PMCID: Pubmed No.:24607296
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells
PMCID: Pubmed No.:24779927
Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells
PMCID: Pubmed No.:25193113
Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
PMCID: Pubmed No.:25213258
Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression
PMCID: Pubmed No.:25394408
Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway
PMCID: Pubmed No.:25564356
Autophagy is involved in ethanol-induced cardia bifida during chick cardiogenesis
PMCID: Pubmed No.:26317250
Proper autophagy is indispensable for angiogenesis during chick embryo development
PMCID: Pubmed No.:27163719
Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells
PMCID: Pubmed No.:25193113
Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
PMCID: Pubmed No.:25213258
Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression
PMCID: Pubmed No.:25394408
Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway
PMCID: Pubmed No.:25564356
Peroxisome proliferator-activated receptor-γ interrupts angiogenic signal transduction via transrepression of platelet-derived growth factor-β receptor in hepatic stellate cells
PMCID: Pubmed No.:24259663
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells
PMCID: Pubmed No.:24779927
Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway
PMCID: Pubmed No.:24366110
Luteolin is effective in the non‐small‐cell lung cancer model with L858R/T790M EGFR mutation and erlotinib resistance
PMCID: Pubmed No.:24471765
Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions
PMCID: Pubmed No.:24504086
Rapamycin attenuates visible light-induced injury in retinal photoreceptor cells via inhibiting endoplasmic reticulum stress
PMCID: Pubmed No.:24607296
Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation
PMCID: Pubmed No.:25751394
Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways
PMCID: Pubmed No.:28433710
Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression
PMCID: Pubmed No.:25394408
Proper autophagy is indispensable for angiogenesis during chick embryo development
PMCID: Pubmed No.:27163719
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes
PMCID: Pubmed No.:26257270
miR-33a-5p enhances the sensitivity of lung adenocarcinoma cells to celastrol by regulating mTOR signaling
PMCID: Pubmed No.:29484434
The Akt pathway mediates the protective effects of myeloid differentiation protein 1 in pathological cardiac remodelling
PMCID: Pubmed No.:34041859
Alogliptin improves endothelial function by promoting autophagy in perivascular adipose tissue of obese mice through a GLP-1-dependent mechanism
PMCID: Pubmed No.:30447331
Neuregulin 4 alleviates hepatic steatosis via activating AMPK/mTOR-mediated autophagy in aged mice fed a high fat diet
PMCID: Pubmed No.:32726654
The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway
PMCID: Pubmed No.:31737114
A novel pathogenic splice site variation in STK11 gene results in Peutz–Jeghers syndrome
PMCID: Pubmed No.:34080793
Functional Characterization of FH Mutation c.557G>A Underlies Uterine Leiomyomas
PMCID: Pubmed No.:35163394
Increased homocysteine regulated by androgen activates autophagy by suppressing the mammalian target of rapamycin pathway in the granulosa cells of polycystic ovary syndrome mice
PMCID: Pubmed No.:35485387
Synthesis of dihydrofuran-3-one and 9,10-phenanthrenequinone hybrid molecules and biological evaluation against colon cancer cells as selective Akt kinase inhibitors
PMCID: Pubmed No.:35751771
Sweroside alleviates hepatic steatosis in part by activating AMPK/mTOR-mediated autophagy in mice
PMCID: Pubmed No.:37269482
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.