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MMP-2 (L638) polyclonal antibody BS1236
  • Western blot (WB) analysis of MMP-2 (L638) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:HuT78 whole cell lysate LaneC:H1792 whole cell lysate LaneD:The Brain tissue lysate of Mouse(40ug) LaneE:The Lung tissue lysate of Mouse(40ug) LaneF:The Brain tissue lysate of Rat(40ug) LaneG:The Lung tissue lysate of Rat(40ug)
  • Immunofluorescence analysis of Hela cells using MMP-2 antibody at dilution of 1:50.
  • Immunofluorescence analysis of Hela cells using MMP-2 antibody at dilution of 1:50.
Product NameMMP-2 (L638) polyclonal antibody
Catalog No.BS1236
Swiss-ProtP08253
Host Rabbit
ReactivityHuman,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 65, 24 kDa
Alternative Name72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1; TBE1; PEX; MMP2; CLG4A
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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100ul $275
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Product Name :
MMP-2 (L638) polyclonal antibody
Background :
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
MMP-2 (L638) polyclonal antibody detects endogenous levels of MMP-2 protein.
Immunogen :
Synthetic peptide, corresponding to Human MMP-2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of MMP-2 (L638) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:HuT78 whole cell lysate LaneC:H1792 whole cell lysate LaneD:The Brain tissue lysate of Mouse(40ug) LaneE:The Lung tissue lysate of Mouse(40ug) LaneF:The Brain tissue lysate of Rat(40ug) LaneG:The Lung tissue lysate of Rat(40ug)
  • Immunofluorescence analysis of Hela cells using MMP-2 antibody at dilution of 1:50.
  • Immunofluorescence analysis of Hela cells using MMP-2 antibody at dilution of 1:50.
Arsenic trioxide inhibits invasion/migration in SGC-7901 cells by activating the reactive oxygen species-dependent cyclooxygenase-2/matrix metalloproteinase-2 pathway. 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PMCID:    Pubmed No.:22895819 Effects of DEHP on endometrial receptivity and embryo implantation in pregnant mice PMCID:    Pubmed No.:23046697 Protective role of metalloproteinase inhibitor (AE-941) on ulcerative colitis in rats PMCID:    Pubmed No.:23323009 The Antioxidant Property of Pachymic Acid Improves Bone Disturbance against AH Plus–induced Inflammation in MC-3T3 E1 Cells PMCID:    Pubmed No.:23522537 Induction of Thoracic Aortic Remodeling by Endothelial-Specific Deletion of MicroRNA-21 in Mice PMCID:    Pubmed No.:23527070 Anti-inflammatory effects of triptolide improve left ventricular function in a rat model of diabetic cardiomyopathy PMCID:    Pubmed No.:23530831 A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells PMCID:    Pubmed No.:23703635 Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition PMCID:    Pubmed No.:23707970 Non-invasive optical imaging of matrix metalloproteinase activity with albumin-based fluorogenic nanoprobes during angiogenesis in a mouse hindlimb ischemia model. PMCID:    Pubmed No.:23773822 CAPN 7 promotes the migration and invasion of human endometrial stromal cell by regulating matrix metalloproteinase 2 activity. PMCID:    Pubmed No.:23855590 HDAC10 suppresses cervical cancer metastasis through inhibition of matrix metalloproteinase (MMP) 2 and 9 expression PMCID:    Pubmed No.:23897811 Epigenetic Silencing of CXCL14 Induced Colorectal Cancer Migration and Invasion PMCID:    Pubmed No.:24099668 MicroRNA-29a promotes colorectal cancer metastasis by regulating matrix metalloproteinase 2 and E-cadherin via KLF4 PMCID:    Pubmed No.:24281002 Abnormal expression of CDK11p58 in prostate cancer PMCID:    Pubmed No.:24397471 IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo PMCID:    Pubmed No.:24528069 Inhibition of metastatic tumor growth and metastasis via targeting metastatic breast cancer by chlorotoxin-modified liposomes PMCID:    Pubmed No.:24559485 Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression PMCID:    Pubmed No.:24608674 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Activation of glucagon‐like peptide‐1 receptor inhibits tumourigenicity and metastasis of human pancreatic cancer cells via PI3K/Akt pathway PMCID:    Pubmed No.:24641303 Granulocyte/Macrophage Colony-Stimulating Factor Influences Angiogenesis by Regulating the Coordinated Expression of VEGF and the Ang/Tie System PMCID:    Pubmed No.:24658178 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Sophoricoside fails the embryo implantation by compromising the uterine endometrial receptivity at implantation “window” of pregnant mice PMCID:    Pubmed No.:24877640 Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling. PMCID:    Pubmed No.:24912879 Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells PMCID:    Pubmed No.:25069680 Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549 PMCID:    Pubmed No.:25539072 DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling PMCID:    Pubmed No.:25520862 Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo PMCID:    Pubmed No.:25201015 Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells PMCID:    Pubmed No.:25527205 Fangchinoline targets PI3K and suppresses PI3K/AKT signaling pathway in SGC7901 cells PMCID:    Pubmed No.:25872479 Epigenetic changes and functional study of HOXA11 in human gastric cancer PMCID:    Pubmed No.:25590359 Downregulation of FOXP3 inhibits invasion and immune escape in cholangiocarcinoma PMCID:    Pubmed No.:25623530 Overexpression of HMGB3 protein promotes cell proliferation, migration and is associated with poor prognosis in urinary bladder cancer patients PMCID:    Pubmed No.:25647262 Angiotensin II increases matrix metalloproteinase 2 expression in human aortic smooth muscle cells via AT1R and ERK1/2 PMCID:    Pubmed No.:25767191 Angiotensin II Induces an Increase in Matrix Metalloproteinase 2 Expression in Aortic Smooth Muscle Cells of Ascending Thoracic Aortic Aneurysms via JNK, ERK1/2 and p38 MAPK Activation. PMCID:    Pubmed No.:25955575 Suppression of motor protein KIF3C expression inhibits tumor growth and metastasis in breast cancer by inhibiting TGF-β signaling PMCID:    Pubmed No.:26272184 Holothurian glycosaminoglycan inhibits metastasis via inhibition of P-selectin in B16F10 melanoma cells PMCID:    Pubmed No.:26318439 Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells PMCID:    Pubmed No.:26550465 Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway. PMCID:    Pubmed No.:26494002 Resveratrol prevents alveolar bone loss in an experimental rat model of periodontitis PMCID:    Pubmed No.:26497626 Intermittent bout exercise training down-regulates age-associated inflammation in skeletal muscles PMCID:    Pubmed No.:26545590 Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer PMCID:    Pubmed No.:26649143 Knocking down Dp71 expression in A549 cells reduces its malignancy in vivo and in vitro PMCID:    Pubmed No.:26691328 (-)-β-hydrastine suppresses the proliferation and invasion of human lung adenocarcinoma cells by inhibiting PAK4 kinase activity PMCID:    Pubmed No.:26821251 Epb41l3 suppresses esophageal squamous cell carcinoma invasion and inhibits MMP2 and MMP9 expression PMCID:    Pubmed No.:26916087 Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages PMCID:    Pubmed No.:27144994 Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549 PMCID:    Pubmed No.:25539072 DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling PMCID:    Pubmed No.:25520862 Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo PMCID:    Pubmed No.:25201015 Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells PMCID:    Pubmed No.:25527205 Epigenetic Silencing of CXCL14 Induced Colorectal Cancer Migration and Invasion PMCID:    Pubmed No.:24099668 MicroRNA-29a promotes colorectal cancer metastasis by regulating matrix metalloproteinase 2 and E-cadherin via KLF4 PMCID:    Pubmed No.:24281002 Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells PMCID:    Pubmed No.:26550465 Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway. PMCID:    Pubmed No.:26494002 Resveratrol prevents alveolar bone loss in an experimental rat model of periodontitis PMCID:    Pubmed No.:26497626 Intermittent bout exercise training down-regulates age-associated inflammation in skeletal muscles PMCID:    Pubmed No.:26545590 Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer PMCID:    Pubmed No.:26649143 Knocking down Dp71 expression in A549 cells reduces its malignancy in vivo and in vitro PMCID:    Pubmed No.:26691328 (-)-β-hydrastine suppresses the proliferation and invasion of human lung adenocarcinoma cells by inhibiting PAK4 kinase activity PMCID:    Pubmed No.:26821251 Epb41l3 suppresses esophageal squamous cell carcinoma invasion and inhibits MMP2 and MMP9 expression PMCID:    Pubmed No.:26916087 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Abnormal expression of CDK11p58 in prostate cancer PMCID:    Pubmed No.:24397471 IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo PMCID:    Pubmed No.:24528069 Inhibition of metastatic tumor growth and metastasis via targeting metastatic breast cancer by chlorotoxin-modified liposomes PMCID:    Pubmed No.:24559485 Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression PMCID:    Pubmed No.:24608674 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Activation of glucagon‐like peptide‐1 receptor inhibits tumourigenicity and metastasis of human pancreatic cancer cells via PI3K/Akt pathway PMCID:    Pubmed No.:24641303 Granulocyte/Macrophage Colony-Stimulating Factor Influences Angiogenesis by Regulating the Coordinated Expression of VEGF and the Ang/Tie System PMCID:    Pubmed No.:24658178 Strategy of STAT3β cell-specific expression in macrophages exhibits antitumor effects on mouse breast cancer PMCID:    Pubmed No.:26181625 provides growth and metastasis advantage for human hepatocellular carcinoma PMCID:    Pubmed No.:28844099 DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling PMCID:    Pubmed No.:25520862 Involvement of Oct4 in the pathogenesis of thoracic aortic dissection via inducing the dedifferentiated phenotype of human aortic smooth muscle cells by directly upregulating KLF5 PMCID:    Pubmed No.:27353340 Methylation of TMEM176A is an independent prognostic marker and is involved in human colorectal cancer development PMCID:    Pubmed No.:28678648 lncRNA H19 regulates epithelial–mesenchymal transition and metastasis of bladder cancer by miR-29b-3p as competing endogenous RNA PMCID:    Pubmed No.:28779971 Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells PMCID:    Pubmed No.:26550465 Dual roles of calpain in facilitating Coxsackievirus B3 replication and prompting inflammation in acute myocarditis PMCID:    Pubmed No.:27472894 PT93, a novel caffeic acid amide derivative, suppresses glioblastoma cells migration, proliferation and MMP-2/-9 expression PMCID:    Pubmed No.:28454354 All-trans retinoic acid inhibits migration, invasion and proliferation, and promotes apoptosis in glioma cells in vitro PMCID:    Pubmed No.:26137156 Resveratrol suppresses human glioblastoma cell migration and invasion via activation of RhoA/ROCK signaling pathway 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attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts PMCID:    Pubmed No.:33759334 Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling PMCID:    Pubmed No.:30405824 Pectin Oligosaccharides Enhance α2,6-Sialylation Modification that Promotes Apoptosis of Bladder Cancer Cells by Targeting the Hedgehog Pathway PMCID:    Pubmed No.:34041669 The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway PMCID:    Pubmed No.:32016456 IL-1β promotes the migration of olfactory epithelium neural stem cells through activating matrix metalloproteinase expressions PMCID:    Pubmed No.:29983220 Downregulation of astrocyte elevated gene‑1 expression inhibits the development of vasculogenic mimicry in gliomas PMCID:    Pubmed No.:33235631 Downregulation of Astrocyte Elevated Gene-1 Expression Combined with 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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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